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ASAKURA Yuki , SEKI Hikaru , MURANAKA Toshiya , YAMAMURA Yoshimi , KUROSAKI Fumiya

… Agrobacterium-mediated transformation of leaf tissues of Atropa belladonna with an adenosine 5'-diphosphate (ADP)-ribosylation factor gene of carrot, arf-001, was performed employing pBCR82 as an expression vector. … Two cell lines of the transformed belladonna were established as the liquid cultures of hairy root tissues, and expression of arf-001 and accumulation of its product in the cells were confirmed by RT-PCR and Western blot analyses, respectively. …

Biological & pharmaceutical bulletin 31(7), 1465-1468, 2008-07-01

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MINO Yoshiki , YUKITA Mayumi , HIRATSUKA Nobuhiro , WARIISHI Hiroyuki

… The complete amino acid sequences of [2Fe-2S] ferredoxin from Atropa belladonna and Hyoscyamus niger have been determined by automated Edman degradation of the entire S-carboxymethylcysteinyl proteins and of the peptides obtained by enzymatic digestion. …

Biological & pharmaceutical bulletin 28(8), 1535-1538, 2005-08-01

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Kato Ryoichi , Arashida Miyuki , Kodama Masahiro , Kamada Hiroshi , Suzuki Takashi

… Hairy roots of Lithospermum erythrorhizon, belladonna and carrot, which were induced by inoculation with Agrobacterium rhizogenes harboring the Ri plasmid, were cultured on a medium containing 0.1, 1, 10, 30 or 100μM W-7 or W-5, calmodulin antagonists. … erythrorhizon and belladonna hairy roots cultured on all media containing W-7 were lower than that of the roots cultured without W-7. …

山形大學紀要. 自然科學 15(3), 79-88, 2003-02-17

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LEE KUNG-TA , HIRANO HIROSHI , YAMAKAWA TAKASHI , KODAMA TOHRU , IGARASHI YASUO , SHIMOMURA KOICHIRO

… The effect of salicylic acid(SA)on tropane alkaloid production and the responses to SA stress of transformed root cultures of Atropa belladonna(belladonna)were investigated. … Treatment of A.belladonna transformed roots with 0.2 mM SA did not have any effect on tropane alkaloid production, but two compounds were produced in the medium. …

Journal of bioscience and bioengineering 91(6), 586-589, 2001-06-25

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Suzuki Ken-ichi , Yamada Yasuyuki , Hashimoto Takashi

… The cDNAs encoding putrescine N-methyltransferase (PMT), which catalyzes the S-adenosylmethionine-dependent N-methylation of putrescine at the first committed step in the biosynthetic pathways of tropane alkaloids, were isolated from Atropa belladonna and Hyoscyamus niger. … AbPMT1 RNA was much more abundant in the root of A.belladonna than was AbPMT2 RNA. …

Plant and cell physiology 40(3), 289-297, 1999-03

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LEE KUNG-TA , YAMAKAWA TAKASHI , KODAMA TOHRU , IGARASHI YASUO , SHIMOMURA KOICHIRO

… Transformed roots of Atropa belladonna strain M8 were cultured in shaking flasks aerated with normal air at 1 vvm, capped with aluminum foil, or capped with a silicon rubber stopper, to investigate the effects of aeration on root growth and on tropane alkaloid production and composition. … In particular, the scopolamine ratio increased from 5.7 to 11.9% of the total alkaloids accumulated in the belladonna cultured roots. …

Journal of fermentation and bioengineering 86(6), 614-616, 1998-12-25

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KANOKWAREE KANOKWAN , DORAN PAULINE M.

… The initial specific growth rate of Atropa belladonna hairy roots in shake flasks was found to vary with the number of inoculum root tips. …

Journal of fermentation and bioengineering 84(4), 378-381, 1997-10-25

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