Search Results:　 1-20 of 239

1

Action of Human Salivary Short Histatins Against The Cell Membrane of Candida Albicans NBRC 1385 [in Japanese]

大滝俊樹 , 笠原仁 , 武井教展 , 谷口正之 , 加藤哲男 , 斎藤英一 , Ohtaki Toshiki , Kasahara Hitoshi , Takei Norinobu , Taniguchi Masayuki , Kato Tetsuo , Saitoh Eiichi

… To study the antifungal mechanism and action against cell membrane of Candida albicans NBRC 1385, we prepared synthetic histatins 5(24 amino acids), 8(12 a.a.), 9(14 a.a.) and 11(8 a.a.). … On the membrane surface, the histatins may not only form the pores by placing their hydrophobic part in contact with the hydrophobic core of membrane but also lead to the collapse of the membrane. …

新潟工科大学研究紀要 (17), 29-34, 2012-12-00

IR
2

Purification, characterization, and primary structure of a novel N-acyl-D-amino acid amidohydrolase from Microbacterium natoriense TNJL143-2(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

Liu Jian , Asano Yu , Ikoma Keiko , Yamashita Satoshi , Hirose Yoshihiko , Shimoyama Takefumi , Takahashi Seiji , Nakayama Toru , Nishino Tokuzo

… A novel N-acyl-D-amino acid amidohydrolase (DAA) was purified from the cells of a novel species of the genus Microbacterium. … It acted on N-acylated hydrophobic D-amino acids with the highest preference for N-acetyl-D-phenylalanine (NADF). … Based on partial amino acid sequences of the purified enzyme, the full-length AcyM gene was cloned and sequenced. …

Journal of bioscience and bioengineering 114(4), 391-397, 2012-10-00

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3

Palatability Characteristics of Pleurotus eryngii var. tuoliensis [in Japanese]

MIYAZAWA Noriko , MATSUOKA Hiroki , OZAWA Yoshio

担子菌バイリング（Pleurotus eryngii var. tuoliensis）を食品栄養学的に位置づける基礎的な知見を得るために，遊離アミノ酸，遊離糖・糖アルコール，有機酸，5′-グアニル酸の分析および官能評価を行った．凍結乾燥試料の遊離アミノ酸含有量は，アルギニン，グルタミン酸，アスパラギン酸，アラニン，ヒスチジ …

Nippon Shokuhin Kagaku Kogaku Kaishi 59(3), 153-160, 2012-03-15

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4

Artificial Protein Assemblies Induced by Metal Coordination Interaction

Oohora Koji

… Most of such assemblies are achieved with electrostatic, hydrophobic and hydrogen bonding interactions. … In contrast, the well-defined protein assembly by the interprotein metal coordination with amino acids has been demonstrated by Tezcan and his group. … Further investigation demonstrated the metal dependent structural changes and stabilization of tetramer by introduction of suitable hydrophobic residues. …

Bulletin of Japan Society of Coordination Chemistry 59(0), 82-83, 2012

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5

Mode of Action of Microbial Anti-MRSA Agents [in Japanese]

Tomoda Hiroshi

… Our computational modeling experiments indicated that spirohexaline A was inserted into the substrate pocket of UPP synthase and interacted with Glu88 via a carbamoyl group of the compound, with Ala76, Met54 and Asn35 via three hydroxyl groups, and with certain hydrophobic amino acids via a spiro ring. …

YAKUGAKU ZASSHI 132(1), 37-44, 2012

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6

Identification of Metal Transporter Genes Using Coding Theory [in Japanese]

Hiroki Sato , Yeondae Kwon , Satoru Miyazaki

グラム陰性桿菌である Stenotrophomonas maltophilia は、抗生物質の β-lactam 環を破壊する β-ラクタマーゼ酵素を産生することで、抗生物質の効果を無力化する。生化学的実験によって、S.maltophilia の薬剤耐性における重金属の取り込みに関連したトランスポーター遺伝子の重金属取り込み機構が、酵素の活性化と深く関係していることが明らかになっている。しかし、ほ …

IPSJ SIG technical reports 2011-BIO-27(11), 1-6, 2011-11-24

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7

Identification of Metal Transporter Genes Using Coding Theory [in Japanese]

Hiroki Sato , Yeondae Kwon , Satoru Miyazaki

グラム陰性桿菌である Stenotrophomonas maltophilia は、抗生物質の β-lactam 環を破壊する β-ラクタマーゼ酵素を産生することで、抗生物質の効果を無力化する。生化学的実験によって、S.maltophilia の薬剤耐性における重金属の取り込みに関連したトランスポーター遺伝子の重金属取り込み機構が、酵素の活性化と深く関係していることが明らかになっている。しかし、ほ …

IPSJ SIG Notes 2011-MPS-86(11), 1-6, 2011-11-24

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8

ASP- and GLU-specific novel dipeptidyl peptidase 11 of Porphyromonas gingivalis that ensures utilization of proteineous energy sources.

Ohara-Nemoto Yuko , Shimoyama Yu , Kimura Shigenobu , Kon Asako , Haraga Hiroshi , Ono Toshio , Nemoto Takayuki K

… The DPP11 gene encoded 717 amino acids with a molecular mass of 81,090, and was present as a 75-kDa form with an N-terminus of Asp22. … DPP11 specifically removed dipeptides from oligopeptides with the penultimate N-terminal Asp and Glu, and has a P2-position preference to hydrophobic residues. …

The Journal of Biological Chemistry 286(44), 38115-38127, 2011-11-04

IR
9

Amino acid components of lees in salmon fish sauce are tyrosine and phenylalanine(BREWING AND FOOD TECHNOLOGY)

Ohmori Taketo , Mutaguchi Yuta , Yoshikawa Shuji , Doi Katsumi , Ohshima Toshihisa

We report that the lees in salmon fish sauce consist of Tyr and Phe. The concentration of free L-Tyr (2.0mM) was almost same as the saturated concentration (2.4mM) in water at 20℃. This result shows t …

Journal of bioscience and bioengineering 112(3), 256-258, 2011-09-00

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10

How Do Cells Sense Mechanical Stresses?(Papers presented at the Seminar, "Biological mechanism of stress perception") [in Japanese]

SAWADA Yasuyuki , SOKABE Masahiro

… We found that MscL senses the tension increase mainly with Phe78 at the lipid-water interface, which induces a subtle change in the channel gate composed of hydrophobic amino acids that stabilizes the closed state of MscL. …

Cryobiology and cryotechnology 57(1), 19-23, 2011-04-15

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11

A functional role of the glycosylated N-terminal domain of chondromodulin-I

KONDO Jun , SHIBATA Hiroyuki , MIURA Shigenori , YAMAKAWA Akira , SATO Koji , HIGUCHI Yoshiki , SHUKUNAMI Chisa , HIRAKI Yuji

… ChM-I comprises two domains: an N-terminal hydrophilic domain (domain 1) containing an N-linked glycosylation site and a C-terminal hydrophobic domain (domain 2) with all four disulfide bonds that are present in this protein. … Although domain 1 is predicted to be hydrophilic per se on the basis of its amino acid sequence, NG-hChM-I remains insoluble in aqueous solution as much as ΔN-hChM-I that lacks the N-terminal 37 amino acids containing an N-glycosylation site. …

Journal of bone and mineral metabolism 29(1), 23-30, 2011-01-30

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12

Synthesis of the glycosaminoglycan-protein linkage tetraosyl peptide moieties of betaglycan, which serve as a hexosamine acceptor for enzymatic glycosyl transfer

Tamura Jun-ichi , Nakamura-Yamamoto Tomomi , Nishimura Yuko , Mizumoto Shuji , Takahashi Jun , Sugahara Kazuyuki

… Although the mechanism behind the assembly of CS and HS is not fully understood, it has been reported that the assembly of HS requires not only a cluster of acidic residues but also hydrophobic residues located near the Ser-Gly attachment sites (Esko, J. …

Carbohydrate Research 345(15), 2115-2123, 2010-10-13

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13

Characterization of polystyrene-binding peptides (PS-tags) for site-specific immobilization of proteins(BIOCHEMICAL ENGINEERING)

Kumada Yoichi , Kuroki Daisuke , Yasui Hidefumi , Ohse Takuhito , Kishimoto Michimasa

… Both the FITC-labeled PS19-1 (RAFIASRRIKRP) and PS19-6 (RIIIRRIRR) peptides showed strong binding affinity for commercially available hydrophilic, but not hydrophobic, PS plates in the presence of the non-ionic surfactant Tween 20. …

Journal of bioscience and bioengineering 109(6), 583-587, 2010-06-00

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14

Mutation analysis of the interaction of B-type cytochrome c oxidase with its natural substrate cytochrome c-551(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

Kabashima Yoshiki , Ueda Naoaki , Sone Nobuhito , Sakamoto Junshi

… Cytochrome bo3-type cytochrome c oxidase from thermophilic Bacillus is a B-type oxidase that is thought to interact with cytochrome c through hydrophobic interactions. … These three residues are located at the front of the hydrophilic globular domain and distant from the CuA site, suggesting that these amino acids compose an acidic patch for a second substrate-binding site. …

Journal of bioscience and bioengineering 109(4), 325-330, 2010-04-00

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15

A Novel Synthesis of N-Alkoxycarbonyl Amino Acids and Surfactant Properties of Their Sodium Salts

Kawase Tokuzo , Nishioka Yukie , Oida Tatsuo

… N-Acyl amino acids have been prepared from fatty acid chlorides and amino acids, but it may not be easy to vary the hydrophobic groups on the view point of availability. …

Journal of Oleo Science 59(4), 191-201, 2010

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16

Identification and Function of Agrin Expressed in Rat Brain Microvessels

KOBAYASHI Hideyuki

脳微小血管に発現しているアグリンのアイソフォームを解析した.ラット大脳皮質から調製した脳微小血管は,N-末が短い(SN)アグリンと,N-末が長い(LN)アグリンの両アイソフォームmRNAを発現しており,LNアグリンが主要アイソフォームであった.ラットLNアグリンのN-末をコードする遺伝子の塩基配列を決定し,N-末には疎水性のクラスターが存在し,分泌のためのシグナル配列と考えられた.アグリンの細胞内 …

Journal of UOEH 31(3), 219-230, 2009-09-01

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17

Properties and substrate specificities of proteolytic enzymes from the edible basidiomycete Grifola frondosa(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

Nishiwaki Toshikazu , Asano Satoshi , Ohyama Takuji

… The ProGF also liberated hydrophobic amino acids, such as valine, leucine, and phenylalanine, using the oxidized insulin B-chain as a substrate. … These results indicate that the ProGF include both endopeptidases recognizing leucine, phenylalanine, and lysine at the P1' position, and aminopeptidases preferentially releasing hydrophobic and aromatic amino acids such as valine, leucine, phenylalanine, and tyrosine. …

Journal of bioscience and bioengineering 107(6), 605-609, 2009-06-00

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18

Determination of three amino acids causing alteration of proteolytic activities of staphylococcal glutamyl endopeptidases.

Nemoto Takayuki K , Ono Toshio , Shimoyama Yu , Kimura Shigenobu , Ohara-Nemoto Yuko

… Expression of chimeric proteins between GluV8 and GluSE revealed that the difference was primarily attributed to amino acids at residues 170-195, which defined the intrinsic protease activity, and additionally to residues 119-169, which affected the proteolysis sensitivity. … but V188 could be replaced by hydrophobic amino acids. …

Biological chemistry 390(3), 277-285, 2009-03-00

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19

Purification, Characterization, and Overexpression of Thermophilic Pectate Lyase of Bacillus sp. RN1 Isolated from a Hot Spring in Thailand

SUKHUMSIIRCHART Wasana , KAWANISHI Sakiko , DEESUKON Warin , CHANSIRI Kosum , KAWASAKI Haruhiko , SAKAMOTO Tatsuji

… The enzyme was purified to homogeneity using cation-exchange and hydrophobic column chromatographies. … gene encoding Pel SWU was 1,023 bp, which corresponds to 341 amino acids. … Unsaturated di- and trigalacturonic acids were formed mainly as the final products of degradation by Pel SWU, as revealed by high-performance anion-exchange chromatography (HPAEC) and electrospray ionization mass spectrometry (ESI-MS) analyses. …

Bioscience, Biotechnology, and Biochemistry 73(2), 268-273, 2009-02-23

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20

Molecular cloning and characterization of the actin-depolymerizing factor gene in Gossypium barbadense

CHI Jina , WANG Xingfen , ZHOU Hongmei , ZHANG Guiyin , SUN Yanxiang , LI Zhikun , MA Zhiying

… A 420 bp open reading frame of the cloned gene, named GbADF1, encoded a protein of 139 amino acids, including 39.57% nonpolar amino acids, 17.27% acidic amino acids, 15.83% basic amino acids and 31.92% hydrophobic amino acids. …

Genes & Genetic Systems 83(5), 383-391, 2008-10-25

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