Search Results:　 1-20 of 26

1

Screening of Furanocoumarin Derivatives in Citrus Fruits by Enzyme-Linked Immunosorbent Assay(Analytical Biochemistry)

SAITA Tetsuya , FUJITO Hiroshi , MORI Masato

… Anti-6',7'-dihydroxybergamottin antibody was obtained by immunizing rabbits with 6',7'-dihydroxybergamottin conjugated with bovine serum albumin using the N-succinimidyl ester method. …

Biological & pharmaceutical bulletin 27(7), 974-977, 2004-07-01

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2

Establishment of an Enzyme-Linked Immunosorbent Assay for Measurement of Sotalol(Analytical Biochemistry)

SAITA Tetsuya , FUJITO Hiroshi , NAKANO Yukitaka , MORI Masato

… Anti-sotalol antibody was obtained by immunizing rabbits with sotalol conjugated with bovine serum albumin using the N-succinimidyl ester method. …

Biological & pharmaceutical bulletin 27(1), 94-96, 2004-01-01

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3

Development of ELISAs for Irinotecan and Its Active Metabolite SN-38

SAITA Tetsuya , FUJITO Hiroshi , MORI Masato

… and specific enzyme-linked immunosorbent assays (ELISAs) for the determination of 7-thyl-10-[4-(1-piperidino)-1-piperidino]carbonyloxy-camptothecin (irinotecan) and 7-ethyl-10-hydroxycamp-tothecin (SN-38), an active metabolite of irinotecan, were developed, which are capable of measuring as low as 16 and 160 pg of each drug/ml, respectively.Anti-irinotecan antibody was obtained by immunizing rabbits with irinotecan conjugated with mercapto-succinyl bovine serum albumin (MS.BSA) using N-(4-diazophenyl)maleimide (DPM) as a heterobifunctional coupling …

Biological & pharmaceutical bulletin 23(8), 911-916, 2000-08-01

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4

Study on Surfactin, a Cyclic Depsipeptide. II. Synthesis of Surfactin B_2 Produced by Bacillus natto KMD 2311

NAGAI Sotoo , OKIMURA Keiko , KAIZAWA Naohito , OHKI Kazuhiro , KANATOMO Shoichi

… Two possible diastereoisomers of surfactin B_2,cyclo[D- and L-3-(Glu-Leu-D-Leu-Val-Asp-D-Leu-Leu-O)-n-tetradecanoyl] (1a and b), were synthesized by a solution method using mainly active ester and azide fragment condensation methods. …

Chemical & pharmaceutical bulletin 44(1), 5-10, 1996-01-15

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5

A Highly Sensitive Enzyme-Linked Immunosorbent Assay (ELISA) for Antitumor Polyacetylenic Alcohol, Panaxytriol

SAITA Tetsuya , MATSUNAGA Hisashi , YAMAMOTO Hiroshi , NAGUMO Fumio , FUJITO Hiroshi , MORI Masato , KATANO Mitsuo

… Anti-panaxytriol antibody was obtained by immunizing rabbits with panaxytriol conjugated with bovine serum albumin using the N-succinimidyl ester method. …

Biological & pharmaceutical bulletin 17(6), 798-802, 1994-06-15

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6

USE OF THE THERMOSTABLE GLUCOSE-6-PHOSPHATE DEHYDROGENASE AS A LABEL ENZYME IN STEROID ENZYME IMMUNOASSAYS

HOSODA Hiroshi , FUKUDA Katsuyuki , TAKAHASHI Noriko , GOTO Junichi

… Enzyme labeling of a steroid with the enzyme was carried out by the N-succinimidyl ester method. …

Chemical & pharmaceutical bulletin 40(1), 294-295, 1992-01-25

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7

Enzyme Labeling in Steroid Enzyme Immunoassays. Comparison of the p-Nitrophenyl Ester and N-Succinimidyl Ester Methods

HOSODA Hiroshi , FUKUDA Katsuyuki , GOTOH Yohko

… Enzyme labeling of steroids by the p-nitrophenyl ester method was investigated in comparison with the N-succinimidyl ester method. … The active ester of a testosterone or 11-deoxycortisol derivative was treated with β-galactosidase and horseradish peroxidase to give labeled antigens. …

Chemical & pharmaceutical bulletin 39(9), 2373-2377, 1991-09-25

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8

Chemistry of Succinimido Esters. XIX:N-Arylacetylation of Amino Groups of Bovine Serum Albumin with N-Succinimidyl Arylacetates

HIRATA Hirofumi , YAMASHINA Takao , HIGUCHI Katsuhiko

… Chemical modification (N-arylacetylation) of amino groups in proteins by N-succinimidyl arylacetates (1a) was studied kinetically using bovine serum albumin (BSA) as the model protein in the range of 7.0≤pH≤9.0 at 20°C. … N-Arylacetylation was competitive with the hydrolysis of (1a). …

Journal of Japan Oil Chemists' Society 40(5), 406-414, 1991

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9

Sensitivity of Steroid Enzyme Immunoassays. Comparison of Four Label Enzymes in an Assay System Using a Monoclonal Anti-steroid Antibody

HOSODA Hiroshi , TSUKAMOTO Reiko , NAMBARA Toshio

… Enzyme labeling of 11-deoxycortisol was carried out by the N-succinimidyl ester method. … The activated ester of 4-(2-carboxyethylthio)-11-deoxycortisol was treated with each enzyme to give a homologous enzyme-labeled antigen. …

Chemical & pharmaceutical bulletin 37(7), 1834-1837, 1989-07-25

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10

UNEXPECTED LOSS OF IMMUNOREACTIVITY OF A GLUCOSE OXIDASE-LABELED ANTIGEN IN A STEROID ENZYME IMMUNOASSAY SYSTEM

HOSODA Hiroshi , ITOH Misuzu , TSUKAMOTO Reiko , NAMBARA Toshio

… The antigen was labeled by the N-succinimidyl ester method. …

Chemical & pharmaceutical bulletin 37(3), 859-860, 1989-03-25

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11

Determination of L-6-Keto-piperidine-2-carbonyl-L-leucyl-L-proline Amide (RGH-2202), a New Analog of Thyrotropin-Releasing Hormone, in Plasma by Radioimmunoassay

TERAUCHI Yoshiaki , ISHIKAWA Shoko , OIDA Sachiko , NEGORO Toshiyuki , KAGEMOTO Akira , SEKINE Yutaka

… An antiserum was produced in guinea pigs immunized with RGH-2202 which was conjugated to bovine serum albumin by an N-succinimidyl ester method. …

Journal of pharmacobio-dynamics 11(6), 459-464, 1988-06-00

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12

Sensitivity of Steroid Enzyme Immunoassays. : Use of 3,3', 5,5'-Tetramethylbenzidine as a Chromogen in the Assay System with Glucose Oxidase Label

HOSODA HIROSHI , TSUKAMOTO REIKO , SHORIKEN KOHICHI , TAKASAKI WATARU , NAMBARA TOSHIO

… The glucose oxidase-labeled antigen was prepared by the N-succinimidyl ester method. …

Chemical & pharmaceutical bulletin 36(5), 1808-1813, 1988-05-25

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13

Enzyme Labeling of Steroids by the N-Succinimidyl Ester Method. Preparation of Glucose Oxidase-Labeled Antigen for Use in Enzyme Immunoassay(Analytical,Chemical)

HOSODA HIROSHI , TSUKAMOTO REIKO , SHISHIDO MAKIKO , TAKASAKI WATARU , NAMBARA TOSHIO

… Enzyme labeling of a steroid with glucose oxidase by the N-succinimidyl ester method was investigated. … The activated ester of a testosterone derivative was treated with glucose oxidase to give a labeled antigen. …

Chemical & pharmaceutical bulletin 35(12), 4856-4861, 1987-12-25

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14

Sensitivity of Steroid Enzyme Immunoassays. Comparison of Alkaline Phosphatase, β-Galactosidase and Horseradish Peroxidase as Labels in a Colorimetric Assay System(Analytical,Chemical)

HOSODA HIROSHI , TAKASAKI WATARU , TSUKAMOTO REIOK , NAMBARA TOSHIO

… Enzyme labeling of testosterone was carried out by the N-succinimidyl ester method at an appropriate molar ratio of steroid to enzyme. …

Chemical & pharmaceutical bulletin 35(8), 3336-3342, 1987-08-25

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15

An Enzyme Immunoassay System with a Monoclonal Antibody for the Determination of 11-Deoxycortisol(Analytical,Chemical)

HOSODA HIROSHI , TAMURA SAKIKO , TSUKAMOTO REIKO , KOBAYASHI NORIHIRO , SAWADA JUN-ICHI , TERAO TADAO , NAMBARA TOSHIO

… The enzyme labeling of 11-deoxycortisol was carried out by the N-succinimidyl ester method. …

Chemical & pharmaceutical bulletin 35(4), 1497-1502, 1987-04-25

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16

Chemistry of Succinimido Esters. V.:Kinetic Studies on the Reactions of N-Succinimidyl Benzoates with Various Amino Acids in Aqueous Solution

HIGUCHI Katsuhiko , ISHIKAWA Kazuhiko , YAMASHINA Takao , HIRATA Hirofumi

… The reactions of N-succinimidyl benzoates (1) with various amino acids (2) to give the corresponding N- (arylcarbonyl) amino acids (3) were studied kinetically at 25°C and pH=pKa. … is the first order rate constant for the N-arylcarbonylation of (2) by (1). …

Journal of Japan Oil Chemists' Society 36(3), 176-182, 1987

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17

A Comparison of Chromogenic Substrates for Horseradish Peroxidase as a Label in Steroid Enzyme Immunoassay

HOSODA HIROSHI , TAKASAKI WATARU , OE TOMOYUKI , TSUKAMOTO REIKO , NAMBARA TOSHIO

… An HRP-labeled antigen was prepared by the N-succinimidyl ester method. …

Chemical & pharmaceutical bulletin 34(10), 4177-4182, 1986-10-25

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18

Bridging Phenomena in Steroid Immunoassays. The Effect of Bridge Length on Sensitivity in Enzyme Immunoassay

HOSODA HIROSHI , KOBAYASHI NORIHIRO , ISHII NORI , NAMBARA TOSHIO

… In order to obtain evidence for the bridge length effect, the sensitivity of an assay system using a β-galactosidase-labeled antigen prepared from an 11-deoxycortisol derivative having a carboxy-methylthio moiety as a bridge at C-4 was compared with that in the case of a derivative with an N-(carboxymethyl)carbamoylmethylthio moiety at C-4. … The enzyme labeling of 11-deoxycortisol was carried out by the N-succinimidyl ester method. …

Chemical & pharmaceutical bulletin 34(5), 2105-2111, 1986-05-25

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19

Enzyme Labeling of Steroids by the N-Succinimidyl Ester Method. Preparation of Alkaline Phosphatase-Labeled Antigen for Use in Enzyme Immunoassay

HOSODA HIROSHI , TAKASAKI WATARU , AIHARA SACHIKO , NAMBARA TOSHIO

… Enzyme labeling of a steroid with alkaline phosphatase by the N-succinimidyl ester method was investigated. … The activated ester of 4-hydroxytestosterone 4-hemiglutarate was treated with alkaline phosphatase to give a labeled antigen. …

Chemical & pharmaceutical bulletin 33(12), 5393-5398, 1985-12-25

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20

Enzyme Labeling of Steroids by the N-Succinimidyl Ester Method. Preparation of Horseradish Peroxidase-Labeled Antigen for Use in Enzyme Immunoassay

HOSODA HIROSHI , KARUBE TOMOKO , KOBAYASHI NORIHIRO , NAMBARA TOSHIO

… Enzyme labeling of a steroid with horseradish peroxidase by the N-succinimidyl ester method has been investigated in comparison with that with β-galactosidase. … The reaction of the activated ester of 4-hydroxytestosterone 4-hemiglutarate with horseradish peroxidase provided a labeled antigen showing high immunoreactivity with an anti-testosterone antiserum in the enzyme immunoassay procedure. …

Chemical & pharmaceutical bulletin 33(1), 249-255, 1985-01-25

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