Search Results:　 1-20 of 4262

1

Interaction site prediction of a peptide which derives from fibronectin and inhibits integrin [in Japanese]

Yosuke Kondo , Yeondae Kwon , Satoru Miyazaki

フィブロネクチンは細胞機能発現のための足場を構築するタンパク質であるが,フィブロネクチンの 22-mer のフラグメント FNIII14 は足場への接着を媒介する分子インテグリンの機能を抑制する.最近,タンパク質翻訳伸長因子 eEF1A(Eukaryotic Elongation Factor 1A) が FNIII14 の細胞膜受容体であることが示唆された.eEF1A は細胞質でペプチド鎖伸長に …

IPSJ SIG technical reports 2011-BIO-27(12), 1-6, 2011-11-24

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2

Interaction site prediction of a peptide which derives from fibronectin and inhibits integrin [in Japanese]

Yosuke Kondo , Yeondae Kwon , Satoru Miyazaki

フィブロネクチンは細胞機能発現のための足場を構築するタンパク質であるが,フィブロネクチンの 22-mer のフラグメント FNIII14 は足場への接着を媒介する分子インテグリンの機能を抑制する.最近,タンパク質翻訳伸長因子 eEF1A(Eukaryotic Elongation Factor 1A) が FNIII14 の細胞膜受容体であることが示唆された.eEF1A は細胞質でペプチド鎖伸長に …

IPSJ SIG Notes 2011-MPS-86(12), 1-6, 2011-11-24

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3

Circulating salmon 28- and 22-kDa insulin-like growth factor binding proteins (IGFBPs) are co-orthologs of IGFBP-1.

Shimizu Munetaka , Kishimoto Keisuke , Yamaguchi Teppei , Nakano Yusuke , Hara Akihiko , Dickhoff Walton W

… In the present study, we cloned the cDNA of 28-kDa IGFBP from Chinook salmon (Oncorhynchus tshawytscha) as well as rainbow trout (Oncorhynchus mykiss) based on the partial N-terminal amino acid sequence of purified protein and identified it as an ortholog of IGFBP-1. …

General and comparative endocrinology 174(2), 97-106, 2011-11-01

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4

cDNA cloning of an alginate lyase from a marine gastropod Aplysia kurodai and assessment of catalytically important residues of this enzyme.

Rahman Mohammad Matiur , Inoue Akira , Tanaka Hiroyuki , Ojima Takao

… However, other gastropod enzymes have not been so well investigated and only partial amino-acid sequences are currently available. … The finally cloned cDNA comprised 1,313 bp which encoded an amino-acid sequence of 295 residues of AkAly30. …

Biochimie 93(10), 1720-1730, 2011-10

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5

cDNA cloning and bacterial expression of an endo-β-1,4-mannanase, AkMan, from Aplysia kurodai

Zahura Umme Afsari , Rahman Mohammad Matiur , Inoue Akira , Tanaka Hiroyuki , Ojima Takao

… kurodai hepatopancreas cDNA using degenerated primers designed on the basis of partial amino-acid sequences of AkMan. … The cDNA including entire translational region of AkMan consisted of 1,392 bp and encoded 369 amino-acid residues. …

Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 159(4), 227-235, 2011-08

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6

The functional effect of Gly209 and Ile213 substitutions on lysozyme activity of family 19 chitinase encoded by cyanophage Ma-LMM01

Hosoda Naohiko , Kurokawa Yoichi , Sako Yoshihiko , Nagasaki Keizo , Yoshida Takashi , Hiroishi Shingo

… whereas lysozymes hydrolyzes peptidoglycan, alternating β-1, 4-linked copolymers of N-acetylmuramic acid (MurNAc) and GlcNAc. … Using amino acid sequence comparison to ORF69, the putative sugar binding residues, Gln162 and Lys165, from the barley chitinase (the model enzyme for the family 19 chitinases) corresponding to subsites −4 and −3 were found to be replaced with Gly209 and Ile213, respectively, in ORF69. …

Fisheries Science 77(4), 665-670, 2011-07

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7

Screening of peptides with a high affinity to bile acids using peptide arrays and a computational analysis(METHODS)

Takeshita Toshikazu , Okochi Mina , Kato Ryuji , Kaga Chiaki , Tomita Yasuyuki , Nagaoka Satoshi , Honda Hiroyuki

… Bile acid binding peptides have attracted attention for the improvement and prevention of hypercholesterolemia. … In this study, screening of bile acid high affinity peptides was investigated using computationally-assisted peptide array analysis. … Starting with the screening data obtained from a limited, random 6-mer library (2212 sequences), the peptides with a high affinity to bile acid were characterized by comparison of high-and low-affinity peptides using fuzzy neural network (FNN) analysis. …

Journal of bioscience and bioengineering 112(1), 92-97, 2011-07

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8

A manual sequence method of peptides and phosphopeptides using 4-(1'-cyanoisoindolyl)phenylisothiocyanate.

Shibata Takayuki , Wainaina Moses N , Miyoshi Takayuki , Kabashima Tsutomu , Kai Masaaki

… A method for sequence analysis and identification of phosphoamino acids in peptides based on high performance liquid chromatography (HPLC) is described. … The peptides were derivatized with an Edman type reagent, 4-(1'-cyanoisoindolyl)phenylisothiocyanate (CIPIC) and subsequently cleaved to generate stable and fluorescent 4-(1'-cyanoisoindolyl)phenylthiazolinone (CIP-TZ)-amino acids. …

Journal of Chromatography. A 1218(24), 3757-3762, 2011-06-17

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9

RNA-DIRECTED MOLECULAR ASYMMETRY OF AMINO ACIDS [in Japanese]

Tamura Koji

… Proteins are synthesized on the ribosome by the elongation of L-amino acids that are attached to tRNAs. … Therefore, aminoacylation of tRNA could be the key step in the origin of amino acid homochirality. … With this in mind, we attempted non-enzymatic aminoacylation of an RNA minihelix (primordial tRNA) with an aminoacyl-phosphate-D-oligonucleotide, which revealed chiral-selective aminoacylation of the RNA minihelix with a clear preference for L-amino acids. …

Viva origino 38(1-4), 18-22, 2011-06-01

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10

Molecular structure and gene analysis of Ce^<3+>-induced methanol dehydrogenase of Bradyrhizobium sp. MAFF211645(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)

Fitriyanto Nanung Agus , Fushimi Mako , Matsunaga Mika , Pertiwiningrum Ambar , Iwama Tomonori , Kawai Keiichi

… The molecular structure and nucleotide sequence of Ce^<3+>-induced methanol dehydrogenase (MDH) of Bradyrhizobium sp. … N-terminal 23-amino acid sequence, 1-NDELHKMAQNPKDWVMPAGDYAN-23, of the purified MDH exhibited 91.3% identity to that of the MDH large subunit-like protein encoded by mxaF' of Bradyrhizobium japonicum USDA110. …

Journal of bioscience and bioengineering 111(6), 613-617, 2011-06

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11

Cloning and optimized expression of a neutral endoglucanase gene (ncel5A) from Volvariella volvacea WX32 in Pichia pastoris(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

Li Jianfang , Tang Cunduo , Shi Hongling , Wu Minchen

… A cDNA fragment encoding a mature neutral endoglucanase with 366 amino acids was cloned from Volvariella volvacea WX32. … Online analysis of amino acid sequence homology showed that the endoglucanase, designated as NCel5A, belongs to glycoside hydrolase family 5. …

Journal of bioscience and bioengineering 111(5), 537-540, 2011-05

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12

Cloning of a thermostable xylanase from Actinomadura sp. S14 and its expression in Escherichia coli and Pichia pastoris(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

Sriyapai Thayat , Somyoonsap Peechapack , Matsui Kenji , Kawai Fusako , Chansiri Kosum

… The deduced amino acid sequence contained a signal peptide of 41 amino acids and a probable mature xylanase of 188 amino acids. …

Journal of bioscience and bioengineering 111(5), 528-536, 2011-05

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13

Selection Dynamic of Escherichia coli Host in M13 Combinatorial Peptide Phage Display Libraries

ZANCONATO Stefano , MINERVINI Giovanni , POLI Irene , DE LUCREZIA Davide

… The effectiveness of any phage display protocol depends directly on library amino acid sequence diversity and the strength of the selection procedure. … The results indicate that sequence censorship exerted by Escherichia coli dramatically reduces library diversity and can significantly impair phage display effectiveness. …

Bioscience, Biotechnology, and Biochemistry 75(4), 812-815, 2011-04-23

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14

Characterization of Chitosanase of a Deep Biosphere Bacillus Strain

KOBAYASHI Tohru , KOIDE Osamu , DEGUCHI Shigeru , HORIKOSHI Koki

To determine the water environment and pollutants in West Java, the contents of metals and general water quality of the Ciliwung River in the Jakarta area were measured. High Escherichia coli number ( …

Bioscience, Biotechnology, and Biochemistry 75(4), 669-673, 2011-04-23

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15

Purification, molecular cloning, and some properties of a manganese-containing superoxide dismutase from Japanese flounder (Paralichthys olivaceus).

Wang Yajun , Osatomi Kiyoshi , Nagatomo Yoshimi , Yoshida Asami , Hara Kenji

… On the other hand, the N-terminal amino acid sequence of this Mn-SOD was determined to be 16 amino acid residues, and the sequence showed high homology to other Mn-SODs but not Japanese flounder Cu,Zn-SOD. …

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 158(4), 289-296, 2011-04

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16

Failure of mycoplasma lipoprotein MALP-2 to induce NK cell activation through dendritic cell TLR2

Sawahata Ryoko , Shime Hiroaki , Yamazaki Sayuri , Inoue Norimitsu , Akazawa Takashi , Fujimoto Yukari , Fukase Koichi , Matsumoto Misako , Seya Tsukasa

… Macrophage-activating lipopeptide 2 (MALP-2), a mycoplasmal diacylated lipopeptide with palmitic acid moiety (Pam2), activates Toll-like receptor (TLR) 2 to induce inflammatory cytokines. … Although synthetic MALP-2 with 6 or 14 amino acids (a.a.) stretch (designated as s and f) matured mDC to induce IL-6, IL-12p40 and TNF-α to a similar extent, they far less activated NK cells than Pam2CSK4, a positive control of 6 a.a.-containing diacyl lipopeptide. …

Microbes and Infection 13(4), 350-358, 2011-04

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17

Cloning and Characterization of the Antiviral Activity of Feline Tetherin/BST-2.

Fukuma Aiko , Abe Masumi , Morikawa Yuko , Miyazawa Takayuki , Yasuda Jiro

… The degree of amino acid sequence identity between human Tetherin/BST-2 and the feline homolog was 44.4%. …

PloS one 6(3), e18247, 2011-03-29

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18

Biochemical and molecular characterization of anthocyanidin/flavonol 3-glucosylation pathways in Rosa×hybrida

FUKUCHI MIZUTANI Masako , AKAGI Misako , ISHIGURO Kanako , KATSUMOTO Yukihisa , FUKUI Yuko , TOGAMI Junichi , NAKAMURA Noriko , TANAKA Yoshikazu

… RhUF3GT1 encoded an amino acid sequence that is almost identical to the reported rose partial UF3GT homologs and highly homologous to strawberry and apple UF3GTs. …

Plant Biotechnology 28(2), 239-244, 2011-03-25

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19

Differential localizations and functions of rice nucleotide pyrophosphatase/phosphodiesterase isozymes 1 and 3

KANEKO Kentaro , YAMADA Chie , YANAGIDA Ai , KOSHU Tsutomu , UMEZAWA Yukiho , ITOH Kimiko , HORI Hidetaka , MITSUI Toshiaki

… The results showed that NPP1 cannot be delivered to the plastid by the N-terminal region, including the ER signal sequence and the proprotein processing site, and that the peptide region, from 308 to 478 amino acid residues, is probably important for the transport of NPP1 into plastids in rice cells. …

Plant Biotechnology 28(1), 69-76, 2011-03-25

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20

Extracellular Laccase Produced by an Edible Basidiomycetous Mushroom, Grifola frondosa : Purification and Characterization

NITHERANONT Thitinard , WATANABE Akira , ASADA Yasuhiko

… The N-terminal amino acid sequence of Lac 1 was highly similar to those of laccases of some other white-rot basidiomycetes. … The Km values of Lac 1 toward 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), 2,6-dimethoxyphenol, guaiacol, catechol, and 3,4-dihydroxy-L-phenylalanine were 0.0137 mM, 0.608 mM, 0.531 mM, 2.51 mM, and 0.149 mM respectively. …

Bioscience, Biotechnology, and Biochemistry 75(3), 538-543, 2011-03-23

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