Search Results:　 1-20 of 1089

1

Effects of Downregulating DNA Methyltransferase 1 Transcript by RNA Interference on DNA Methylation Status of the Satellite I Region and In Vitro Development of Bovine Somatic Cell Nuclear Transfer Embryos

YAMANAKA Ken-ichi , SAKATANI Miki , KUBOTA Kaiyu , BALBOULA Ahmed Zaky , SAWAI Ken , TAKAHASHI Masashi

… First, the levels of DNMT1 expression were analyzed at 0, 24, 48, 72, 120 and 192 h after in vitro culture. … Real-time PCR and western blotting analyses detected a significant decrease in DNMT1 mRNA in the siRNA-injected NT (siRNA-NT) group up to 72 h after in vitro culture. … Next, the levels of DNA methylation of the satellite I region were analyzed at several time points after in vitro culture. …

Journal of Reproduction and Development 57(3), 393-402, 2011-06-01

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2

The Effects of Manipulation Medium, Culture System and Recipient Cytoplast on In Vitro Development of Intraspecies and Intergeneric Felid Embryos

IMSOONTHORNRUKSA Sumeth , LORTHONGPANICH Chanchao , SANGMALEE Anawat , SRIRATTANA Kanokwan , LAOWTAMMATHRON Chuti , TUNWATTANA Wanchai , SOMSA Wachiravit , KETUDAT-CAIRNS Mariena , NAGAI Takashi , PARNPAI Rangsun

… These results demonstrated that EHM was superior to 199H as an embryo manipulation medium and that the DCo and Bo could support the early embryonic development of intergeneric cloned marbled cat embryos up to the morula stage. …

Journal of Reproduction and Development 57(3), 385-392, 2011-06-01

J-STAGE CrossRef References (43)
3

The Use of Embryonic Stem Cell Derived Bioactive Material as a New Protein Supplement for the In Vitro Culture of Bovine Embryos

KIM Eun Young , LEE Jun Beom , PARK Hyo Young , JEONG Chang Jin , RIU Key Zung , PARK Se Pill

… Components derived from ES cells may contain various bioactive materials (BM) helpful for early preimplantation embryo growth. … In this study, we examined the effect of human ES cell derived BM (hES-BM) on in vitro culture of bovine embryos. … When bovine parthenogenetic day 2 embryos were cultured in 10% hES-BM, a significantly higher embryo development rate (44.3%) and increased cell numbers were observed relative to control medium containing 3 mg/ml BSA (19.5%; …

Journal of Reproduction and Development 57(3), 346-354, 2011-06-01

J-STAGE CrossRef References (32)
4

In vitro Culture of Testicular and Ovarian Gonocytes Obtained from 19-day Incubated Chicken Embryos and Subsequent Colonization into Gonads of Recipient Embryos

NAITO Mitsuru , HARUMI Takashi , KUWANA Takashi

Journal of poultry science 48(2), 112-118, 2011-04-25

References (28)
5

Respiration activity of bovine embryos produced by a single embryo culture system [in Japanese]

KAITO Kohei , TAKAKURA Kei , ABE Yasuyuki , ABE Hiroyuki

Journal of mammalian ova research = 日本哺乳動物卵子学会誌 28(2), 90, 2011-04-01
6

Improvement of microspore culture method for multiple samples in Brassica

TAKAHASHI Yu , YOKOI Shuji , TAKAHATA Yoshihito

… Microspore culture is an important method for production of haploids and doubled haploids. … Although the routine protocol of isolated microspore culture of Brassica species has been established, the protocol for a large number of genotypes is laborious. … In this paper, we report an improvement of the microspore culture method for dealing with multiple samples. …

Breeding science 61(1), 96-98, 2011-03-01

IR References (13)
7

Possible mechanism for developmental toxicity and endocrine disrupting activity induced by benomyl and carbendazim with rat embryo culture(Ecotoxicology,Poster,2) Environmental Fate and Safety Assessment) [in Japanese]

Chang C. W. , Hsu Y. H. , Yuan C. Y. , Liou Y. T. , Chen M. C. , Chen J. H. , Lu S. Y. , Hsu J. C.

Journal of pesticide science 36(1), 181, 2011-02-20

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8

Development and Application of a Chemically Defined Medium for the In Vitro Production of Porcine Embryos

YOSHIOKA Koji

… Recent advances in systems for in vitro production (IVP) of porcine embryos, including in vitro oocyte maturation, fertilization and embryo culture, have enabled us to generate viable embryos that can develop to full term after transfer into recipients. … This technology is being applied now to developments in gamete/embryo biology and agriculture, as well as in producing cloned and genetically modified pigs. …

Journal of Reproduction and Development 57(1), 9-16, 2011-02-01

J-STAGE CrossRef References (74)
9

Gene Silencing of Tead3 Abrogates Radiation-induced Adaptive Response in Cultured Mouse Limb Bud Cells

VARES Guillaume , WANG Bing , TANAKA Kaoru , SHANG Yi , TAKI Keiko , NAKAJIMA Tetsuo , NENOI Mitsuru

… We previously described AR-specific gene modulations in the mouse embryo. … In this study, we evaluated the role of three candidate genes in the apoptotic AR in a micromass culture of limb bud cells: Csf1, Cacna1a and Tead3. …

Journal of Radiation Research 52(1), 39-46, 2011-01-16

J-STAGE Ichushi Web CrossRef References (43)
10

The Effect of Sevoflurane on the Incidence of Cleft Lip in Developing A/J Mouse Embryos [in Japanese]

山田守正

愛知学院大学歯学会誌 49(3), 285-298, 2011-09
11

Embryo Development and the Mechanisms behind the Improvements in In Vitro Dynamic Culture Systems [in Japanese]

松浦宏治 , 成瀬恵治

Journal of mammalian ova research 28(4), 174-179, 2011-10
12

Normal Calves Produced After Transfer of Embryos Cultured in a Chemically Defined Medium Supplemented with Epidermal Growth Factor and Insulin-like Growth Factor I Following Ovum Pick Up and In Vitro Fertilization in Japanese Black Cows

SAKAGAMI Nobutada , UMEKI Hidenobu , NISHINO Osamu , UCHIYAMA Hiroko , ICHIKAWA Kyoko , TAKESHITA Kazuhisa , KANEKO Etsushi , AKIYAMA Kiyoshi , KOBAYASHI Shuji , TAMADA Hiromichi

… The objective of this study was to examine whether high concentrations of epidermal growth factor (EGF) and/or insulin-like growth factor I (IGF-I) would have a beneficial effect on bovine embryo development in vitro and to obtain normal calves by using an ovum pick up method and embryo culture in a chemically defined medium. …

Journal of Reproduction and Development advpub(0), 1111070412, 2011

J-STAGE CrossRef
13

Live Offspring from Cryopreserved Embryos Following In Vitro Growth, Maturation and Fertilization of Oocytes Derived from Preantral Follicles in Mice

MOTOHASHI Hideyuki H. , SANKAI Tadashi , KADA Hidemi

… An oocyte culture system for IVG and IVM was used in oocyte-granulosa cell complexes (OGCs) derived from preantral follicles in 12-day-old mice. … The rate of oocyte maturation was improved by the addition of gonadotropins (FSH/LH) and cytokines (IGF-I/SCF) to culture medium for IVG. … During culture for IVG, estradiol-17β and progesterone concentrations increased progressively to the latter period of culture. …

Journal of Reproduction and Development advpub(0), 1109130399, 2011

J-STAGE CrossRef
14

Establishment of an Advanced Chemically Defined Medium for Early Embryos Derived from In Vitro Matured and Fertilized Bovine Oocytes

MOMOZAWA Kenji , FUKUDA Yoshinori

… A chemically defined medium would be useful for analyzing promoters or inhibitors in in vitro culture (IVC) of bovine embryos. … The present study was carried out to establish an advanced chemically defined medium for bovine embryos that supports a high rate of embryo development to the blastocyst stage. …

Journal of Reproduction and Development advpub(0), 1107280392, 2011

J-STAGE CrossRef
15

In-straw Cryoprotectant Dilution for Bovine Embryos Vitrified using Cryotop

INABA Yasushi , AIKAWA Yoshio , HIRAI Tomokazu , HASHIYADA Yutaka , YAMANOUCHI Tadayuki , MISUMI Koji , OHTAKE Masaki , SOMFAI Tamas , KOBAYASHI Shuji , SAITO Norio , MATOBA Satoko , KONISHI Kazuyuki , IMAI Kei

… The aim of this study was to develop an in-straw dilution method suitable for 1-step bovine embryo transfer of vitrified embryos using the Cryotop vitrification-straw dilution (CVSD) method. …

Journal of Reproduction and Development advpub(0), 1104010362, 2011

J-STAGE CrossRef
16

Impact of Heat Stress Exposure during Meiotic Maturation on Oocyte, Surrounding Cumulus Cell, and Embryo RNA Populations

PAYTON Rebecca R. , RISPOLI Louisa A. , SAXTON Arnold M. , EDWARDS J. Lannett

… Culture at 41 C for the first 12 h of meiotic maturation had no impact on RNA size distribution or transcripts examined from oocytes, surrounding cumulus or resultant 4 to 8-cell embryos. …

Journal of Reproduction and Development advpub(0), 1104050365, 2011

J-STAGE CrossRef
17

A Self-Contained Culture Platform Using Carbon Dioxide Produced from a Chemical Reaction Supports Mouse Blastocyst Development In Vitro

SWAIN Jason E.

… Elevated CO2 is required for in vitro embryo culture to maintain proper media pH and to supply embryo metabolic pathways. … A closed culture system was constructed and chemicals added to generate CO2, which was then supplied to development embryos. … This approach provides a highly purified, inexpensive, and easily obtainable gas source and offers potential for development of new, self-contained culture platforms. …

Journal of Reproduction and Development advpub(0), 1104260370, 2011

J-STAGE CrossRef
18

Study on fertilization and embryo culture in without albumin HTF medium from using L-carnitine [in Japanese]

東佳澄 , 西村愛美 , 木我敬太 [他]

… [Abstract] The present study examined in vitro fertilization that used the ICR mouse and the in vitro culture of the early embryo. …

Memoirs of Institute of Advanced Technology, Kinki University (16), 43-50, 2011-03

IR
19

Synergistic Effects of Glutathione and β-Mercaptoethanol Treatment During In Vitro Maturation of Porcine Oocytes on Early Embryonic Development in a Culture System Supplemented with L-cysteine

CHOE Changyong , SHIN Yong-Won , KIM Eun-Jin , CHO Sang-Rae , KIM Hyun-Jong , CHOI Sun-Ho , HAN Man-Hye , HAN Jaehee , SON Dong-Soo , KANG Dawon

The Journal of reproduction and development 56(6), 575-582, 2010-12-01

Ichushi Web References (44)
20

Regulation of Angiogenesis and Decidualization by Sex Steroid Hormones in Human Endometrium [in Japanese]

OKADA Hidetaka

… Proprotein convertase (PC) 6 is suggested to play an important role in the processes of stromal cell decidualization and embryo implantation in the mouse. … The decrease in levels of free VEGF was proportionate to the rise in sVEGFR-1 levels in the culture media of ESCs. …

Acta Obstetrica et Gynaecologica Japonica 62(11), 2337-2349, 2010-11-01

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