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HASEGAWA Ayumi , YONEZAWA Kazuya , OHTA Akihiko , MOCHIDA Keiji , OGURA Atsuo

… In this study, we sought to develop a new protocol for sperm freezing using cryotubes as the container to increase the accessibility of mouse sperm cryopreservation. … Epididymal spermatozoa were collected from mature ICR or C57BL/6J (B6) males and were suspended in 18% raffinose and 3% skim milk solution. …

Journal of Reproduction and Development advpub(0), 1110260404, 2011

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TOYONAGA Mari , KAIHARA Aya , TSUTSUI Toshihiko

… On the assumption that animals of wild feline species died in the field, caudal epididymal sperm were cryopreserved following storage of the feline epididymides at 20℃ for 0–24 hr, and their qualities were observed. … On comparison of the qualities between caudal sperm cryopreserved after 24 hr storage at 4℃ and after 12 hr at 20℃ followed by 12 hr storage at 4℃, no significant differences were noted. …

Journal of Veterinary Medical Science advpub(0), 1106110546, 2011

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木我 敬太 , 栗田 佳織 , 西村 愛美 [他]

… 精子細胞膜損傷はLIVE/DEAD sperm viability kit を用いて評価し、R18S3 とR18+AFP IIIは5日目の細胞膜完全性の保持率は共に48％であった。 … [Abstract] The present study was performed to examine the storage of Slc : B6D2F1 mouse epididymal sperm at cold temperature. … Afterwards, the evaluation and the in vitro fertilization operation of the refrigeration sperm were examined. …

近畿大学先端技術総合研究所紀要 (16), 51-58, 2011-03

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HUO Shuying , XU Zhihao , ZHANG Xiaoxin , ZHANG Jianfang , CUI Sheng

… We transected the SSNs and ISNs of rats on postnatal day (PD) 30 and then analyzed changes in testicular morphology and cauda epididymal sperm content, cell proliferation and apoptosis and primary spermatocyte meiosis on PD60 and PD90. … The results demonstrated that testicular denervation significantly reduced testis mass, cauda epididymal sperm counts and serum testosterone concentrations. …

The Journal of reproduction and development 56(4), 370-378, 2010-08-01

J-STAGE 医中誌 CrossRef 参考文献49件

TOYONAGA Mari , SATO Yuuka , MORITA Miki , WATANABE Masashi , OBA Hiromichi , MIZUTANI Tatsuji , HORI Tatsuya , TSUTSUI Toshihiko

… We observed the influences of low-temperature storage of the feline epididymis on the epididymal semen qualities before and after cryopreservation to identify the optimal duration for low-temperature storage of the epididymis. … After excision, the feline epididymis was stored at 4°C for 0-72 hr and then subjected to epididymal sperm collection. …

Journal of Veterinary Medical Science 72(6), 777-780, 2010

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伊藤 慧 , 岸 昌生 , 西村 愛美 [他]

… 精子頭部における膜損傷の有無をLIVE/DEAD Sperm Viability Kit を用いて評価し、2：1 稀釈液において7 日目の正常膜保持精子率は40％であった。 …

近畿大学先端技術総合研究所紀要 (15), 17-25, 2010-03

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GOTO Namiko , HARAYAMA Hiroshi

… Protein serine/threonine phosphorylation in mammalian sperm flagella has been considered to play important roles in regulation of motility. … The aims of this study were to disclose roles of protein phosphatases in the regulation of sperm motility and to provide evidence for suppression of PKA full activation by protein phosphatases in sperm flagella. …

The Journal of reproduction and development 55(3), 327-334, 2009-06-01

J-STAGE 医中誌 CrossRef 参考文献56件 被引用文献1件

HORI Tatsuya , UEHARA Yuusuke , KAWAKAMI Eiichi , TSUTSUI Toshihiko

… The effects of the time period between canine epididymis removal and cooling on post-thaw caudal epididymal sperm quality were investigated. … Sperm recovered from the epididymis stored for 6 hr at 4 or 20°C exhibited similar motility. … However, when the epididymis was stored for 12 hr or longer at 20°C, sperm motility was significantly lower than that at 4°C (p<0.01). …

Journal of Veterinary Medical Science 71(6), 811-815, 2009

J-STAGE 医中誌 CrossRef

立野裕幸

… authorThe occurrence of structural chromosome aberrations in mouse one-cell embryos produced by intracytoplasmic sperm injection (ICSI) with mature spermatozoa was dependent on the type of sperm incubation medium and sperm incubation time. …

Mutation Research : Genetic Toxicology and Environmental Mutagenesis 657(1), 26-31, 2008-17-Nov

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AMARA Salem , ABDELMELEK Hafedh , GARREL Catherine , GUIRAUD Pascale , DOUKI Thierry , RAVANAT Jean-Luc , FAVIER Alain , SAKLY Mohsen , BEN RHOUMA Khemais

… -48%, p<0.05), epididymal sperm count (-22%, p<0.05), and spermatozoa motility (-35%, p<0.05). … Interestingly, zinc supplementation attenuated DNA oxidation induced by Cd in the gonad and restored the testosterone level and sperm count to the levels of the control group. …

The Journal of reproduction and development 54(2), 129-134, 2008-04-01

J-STAGE 医中誌 CrossRef 参考文献44件