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Development of a strain for efficient degradation of polychlorinated biphenyls by patchwork assembly of degradation pathways(ENVIRONMENTAL BIOTECHNOLOGY)
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Ohmori Tsuneo
,
Morita Hirokazu
,
Tanaka Megumi
,
Miyauchi Keisuke
,
Kasai Daisuke
,
Furukawa Kensuke
,
Miyashita Kiyotaka
,
Ogawa Naoto
,
Masai Eiji
,
Fukuda Masao
… The resulting recombinant strain exhibited no biphenyl 2,3-dioxygenase (BphA) activity encoded by bphAaAbAcAd genes, which encode the large and small subunits of the terminal oxygenase component and the ferredoxin and reductase subunits responsible for electron transfer from NADH to the large subunit. … To obtain the BphA activity of RHA1 in NK8, sets of BphA genes were constructed by combining the bphAaAbAcAd genes of RHA1 and bphA3A4 of Pseudomonas pseudoalcaligenes KF707, encoding the ferredoxin and reductase subunits. …
Journal of bioscience and bioengineering 111(4), 437-442, 2011-04
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