Search Results:　 1-20 of 151

1

Development of a Competitive ELISA for the Detection of a Furaltadone Marker Residue, 3-Amino-5-Morpholinomethyl-2-Oxazolidinone (AMOZ), in Cultured Fish Samples

SHEU Shi-Yuan , TAI Yung-Te , LI Wen-Ren , LEI Yi-Chih , HSIEH Kuan-Huei , LIN Chiu-Yueh , CHENG Chu-Chen , CHANG Tong-Hsuan , KUO Tzong-Fu

… Polyclonal rabbit antibodies were produced with a new immunogen hapten, 2-NP-HXA-AMOZ. …

Journal of Veterinary Medical Science 74(11), 1439-1446, 2012

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2

Immunoprotection of Japanese Flounder Paralichthys olivaceus by Recombinant Vibrio anguillarum Hemolysin

WANG Hong-Bin , JIAO Yu-Liang , XU Jia-Tao , LI Xin-Shu , XU Bing , DONG Dan , YAN Bin-Lun

V. anguillarumヘモリジン遺伝子vah4の組換えタンパク質VAH4をヒラメに注射し、その免疫効果を調べた。VAH4接種魚の肝臓中のSOD、CAT、AKPおよびACP活性は、対照魚に比べて上昇し、72時間後には同レベルとなった。接種10日後の血清のVAH4に対するELlSA抗体価は1：10、240であった。V. anguillarumの攻撃試験では、対照魚の生存率が0％であったのに対し、 …

Fish Pathology 46(1), 19-22, 2011-03-15

Japanese Agricultural Sciences Index J-STAGE CrossRef References (16)
3

Expression and Immunological Studies of Classical Swine Fever Virus Glycoprotein E2 in the Bi-Cistronic Baculovirus/Larvae Expression System

WU Chi-Ming , HSUAN Shih-Ling , CHEN Zeng-Weng , JINN Tzyy-Rong , HUANG Chienjin , LIAO Jiunn-Wang , CHEN Ter-Hsin , LIAO Chih-Ming , LEE Wei-Cheng , WU Tzong-Yuan , CHIEN Maw-Sheng

… To develop an economical, easy technique for producing recombinant E2 glycoprotein (rE2) of classical swine fever virus (CSFV) as a candidate immunogen, a bi-cistronic baculovirus/larvae expression vector was constructed using p10 …

Bioscience, Biotechnology, and Biochemistry 74(7), 1343-1349, 2010-07-23

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4

Detection of Cephalopod Allergen in Food Products by ELISA [in Japanese]

SHIBAHARA Yusuke , UESAKA Yoshihiko , ABE Akihisa , YAMADA Shoichi , USHIO Hideki , SHIOMI Kazuo

スルメイカ精製トロポミオシンを免疫原として，頭足類トロポミオシンに特異的に反応するモノクローナル抗体を作製し，頭足類トロポミオシン測定用のサンドイッチELISA法を確立した．本法では，頭足類に分類されるいか類，たこ類のトロポミオシンとは全般的に反応したが，甲殻類および貝類トロポミオシンとの交差率は0.1％未満であった．また，食品全般においても頭足類以外で反応は認められなかった．検出感度は頭足類総タ …

Nippon Shokuhin Kagaku Kogaku Kaishi 57(5), 198-204, 2010-05-15

Japanese Agricultural Sciences Index J-STAGE CrossRef References (17)
5

Determination of Crustacean Allergen in Food Products by Sandwich ELISA [in Japanese]

Shibahara Yusuke , Oka Michihiro , Tominaga Kei , Ii Toshihiro , Umeda Mamoru , Uneo Noriko , Abe Akihisa , Ohashi Eiji , Ushio Hideki , Shiomi Kazuo

ブラックタイガー由来精製トロポミオシンを免疫原として，甲殻類トロポミオシンに特異的に反応するモノクローナル抗体を作製し，甲殻類トロポミオシン測定用のサンドイッチELISA法を確立した．本法では，甲殻類に分類されるえび類，かに類，やどかり類，おきあみ類のトロポミオシンとは交差率82～102％と全般的に反応したが，軟体動物に分類されるいか類，たこ類，貝類トロポミオシンとの交差率は0.1％ …

Nippon Shokuhin Kagaku Kogaku Kaishi 54(6), 280-286, 2007

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6

Development of a Monoclonal Antibody-Based Sandwich ELISA for Detection of Guinea Pig Interleukin-2(Clinical Pathology)

ADACHI Satoshi , HASHIMOTO Tomomi , TAKEYOSHI Masahiro , KATO Hirotomo , IWATA Hiroyuki

モルモットにおける免疫応答解析のための基礎研究として,免疫応答の中心的な役割を担うサイトカインであるIL-2に対するモノクローナル抗体を作製し,それらを用いたサンドイッチELISAによるモルモットIL-2 (GPIL-2)定量系の確立を行った.組換えGPIL-2を大腸菌発現系において発現・精製し,これを免疫原に用いて抗GPIL-2モノクローナル抗体の作製を行ったところ,3クローンの抗GPIL-2抗 …

The journal of veterinary medical science 68(12), 1281-1287, 2006-12-25

CiNii Fulltext PDF - Open Access Japanese Agricultural Sciences Index Ichushi Web References (21)
7

Calreticulin is Transiently Induced after Immunogen Treatment in the Fat Body of the Silkworm Bombyx mori

TAKAHASHI Tadashi , MURAKAMI Hiroki , IMANISHI Shigeo , MIYAZAKI Masao , KAMIIE Katsuyoshi , SUZUKI Koichi , TAIRA Hideharu , YAMASHITA Tetsuro

Journal of insect biotechnology and sericology 75(2), 79-84, 2006-06-30

References (20) Cited by (3)
8

Low-dose intraperitoneal Freund's adjuvant : toxicity and immunogenicity in mice using an immunogen targeting amyloid-beta peptide

OSCHERWITZ J.

Vaccine 24, 3018-3025, 2006

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9

Development of a Monoclonal Antibody-Based Sandwich ELISA for Detection of Guinea Pig Interleukin-2

ADACHI Satoshi , HASHIMOTO Tomomi , TAKEYOSHI Masahiro , KATO Hirotomo , IWATA Hiroyuki

… coli) and by using this protein as an immunogen, monoclonal antibodies (mAbs) against GPIL-2 were produced to establish a basis for a research on immune responses in guinea pigs. …

Journal of Veterinary Medical Science 68(12), 1281-1287, 2006

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10

DIETARY INSULIN AS AN IMMUNOGEN AND TOLEROGEN

TIITTANEN Minna , PARONEN Johanna , SAVILAHTI Erkki , VIRTANEN Suvi M. , ILONEN Jorma , KNIP Mikael , AKERBLOM Hans K. , VAARALA Outi , the Finnish TRIGR Study Group

Endocrine journal 52, 79, 2005-10-01

Ichushi Web
11

On the Relationship Between the Structure of Immunogen and Affinity of Raised Antibody for Nonylphenol [in Japanese]

Matsumoto Kiyoshi , Sinohara Katsura , Sakai Takatoshi

… When NP was measured using antibody raised with the conjugate as an immunogen by an indirect competitive ELISA, the antibody showed an affinity with NP, but the magnitude of the affinity was weak. …

Science bulletin of the Faculty of Agriculture, Kyushu University 60(2), 213-222, 2005-10-00

Japanese Agricultural Sciences Index
12

Application of tick-borne encephalitis virus recombinant proteins to ELISA antigen and immunogen.(Hokkaido University confered the degree of Bachelor of Veterinary Medicine to the following 40 graduates of the School of Veterinary Medicine on March 25, 2005.)

Kawakami Kazue

Japanese journal of veterinary research 53(1-2), 100-101, 2005-08-31

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13

The Recombinant Immunogen with High-Density Epitopes of ELDKWA and ELDEWA Induced Antibodies Recognizing Both Epitopes on HIV-1 gp41

WANG Zuguang , LIU Zuqiang , CHENG Xiwen , CHEN Ying-Hua

Microbiology and Immunology 49(8), 703-709, 2005-08-20

Ichushi Web References (17)
14

Induction of Interferon-Gamma (IFN-γ) and T Helper 1 (Th1) Immune Response by Bitter Gourd Extract(Immunology)

IKE Kazunori , UCHIDA Yuko , NAKAMURA Tomohiko , IMAI Soichi

野菜乳剤投与マウスの血中インターフェロン(IFN)-γおよびインターロイキン(IL)-4の誘導を検討した結果, IL-4を顕著に誘導するものはなかったが蔓茘枝乳剤に高いIFN-γ誘導能が認められた.さらに蔓茘枝の果肉, ワタおよび種子乳剤を検討した結果, ワタ乳剤には強いIFN-γ誘導物質が含有されていた.また蔓茘枝ワタ加熱抽出物を含む免疫原によるマウス誘導試験を行った結果, 蔓茘枝ワタ抽出物は高 …

The journal of veterinary medical science 67(5), 521-524, 2005-05-25

CiNii Fulltext PDF - Open Access Japanese Agricultural Sciences Index Ichushi Web References (21)
15

Production and Characterization of Monoclonal Antibodies against Porcine Interleukin-4(Clinical Pathology)

ISHIKURA Yoji , KATO Hirotomo , HASHIMOTO Tomomi , NISHIMURA Yorihiro , IWATA Hiroyuki

ブタにおけるTh細胞の分類およびその機能解析, さらには産生サイトカインの定量法確立の一助とすることを目的に, 組み換えブタIL-4(pIL-4)を大腸菌発現系において発現・精製し, これを免疫原に用いて抗pIL-4モノクローナル抗体の作製を行った.その結果, 最終的に6クローンの抗pIL-4抗体陽性ハイブリドーマが得られ, これらのハイブリドーマから産生されるモノクローナル抗体の免疫グロブリンの …

The journal of veterinary medical science 67(5), 503-508, 2005-05-25

CiNii Fulltext PDF - Open Access Japanese Agricultural Sciences Index J-STAGE Ichushi Web CrossRef References (20)
16

Preparation of Functional Single-Chain Antibodies against Bioactive Gibberellins by Utilizing Randomly Mutagenized Phage-Display Libraries

SUZUKI Yoshihito , ITO Shinsaku , OTSUKA Kaori , IWASAWA Eriko , NAKAJIMA Masatoshi , YAMAGUCHI Isomaro

… were successfully obtained by successive rounds of biopanning against BSA-GA4, the original immunogen. …

Bioscience, Biotechnology, and Biochemistry 69(3), 610-619, 2005-03-23

J-STAGE CrossRef References (14) Cited by (2)
17

PRODUCTION MONOCLONAL ANTIBODIES AGAINST DEOXYNIVALENOL AND DEVELOPMENT OF A HIGH SENSITIVE ELISA USING ENZYME AMMPLIFICATION [in Japanese]

Kawamura Osamu

DON-HG-BSAで免役したマウスの脾臓細胞とミエローマ細胞を融合することにより、5つの抗DONモノクローナル抗体産生ハイブリドーマ（DON、１-５）を樹立した。HRP／MBZ法において、DON.1とDON.3抗体を用いた場合100ng／mlのDONの検出が可能であった。また、酵素サイクリング法を導入したALP／NADP法では、5ng／mlのDONの検出が可能であり、20倍の高感度化に成功した。 …

Technical bulletin of Faculty of Agriculture, Kagawa University 57, 27-33, 2005-02-15

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18

Generation of high performance anti-hapten monoclonal antibodies and their application to trace characterization [in Japanese]

Kobayashi Norihiro , Goto Junichi , Shimada Kazutake , Matsuki Yasuhiko , Kato Yoshinori

… However, it is still not very easy to establish practical monoclonal anti-hapten antibodies, because this establishment requires an adequate cell culture, a reasonable design of hapten-carrier conjugate (immunogen), an effective immunization, an efficient antibody screening in hybridoma microcultures, etc. …

Japanese Journal of Clinical Chemistry 34(2), 125-145, 2005

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19

Induction of Interferon-Gamma (IFN-.GAMMA.) and T Helper 1 (Th1) Immune Response by Bitter Gourd Extract

IKE Kazunori , UCHIDA Yuko , NAKAMURA Tomohiko , IMAI Soichi

… Trial immunogen including the heat extract of the pulp induced specific IgG2a … antibody of the mice serum inoculated with this immunogen. …

Journal of Veterinary Medical Science 67(5), 521-524, 2005

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20

Studies on efficacy of recombinant R7 protein as immunogen for vaccine and as antigen for detection of antibody against Leucocytozoon caulleryi infection in chickens

ITO Akira

The bulletin of the Nippon Veterinary and Zootechnical College 53, 53-54, 2004-12-01

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