Search Results:　 1-20 of 103

Kusakari Ken , Yokoyama Mineyuki , Inomata Shinji , Gozu Yoko , Katagiri Chika , Sugimoto Yukihiro

… In contrast, efficient aeration through a ceramic sparger placed at the bottom of a 20-L bubble column reactor yielded approximately 25g/L of dry roots and 500mg/L of saikosaponin-a and saikosaponin-d over 42 days. … This configuration was successfully applied at the 200-L scale, yielding 500-600mg/L of saikosaponin-a and saikosaponin-d over 56 days. …

Journal of bioscience and bioengineering 113(1), 99-105, 2012-01-00

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Shon Tae Kwon , Zhu Shu-hang , Lee Sang Chul , Shoyama Yukihiro , Tanaka Hiroyuki

… This study showed the effective microanalytic methods for detection of saikosaponin by a competitive enzyme-linked immunosorbent assay (ELISA) using anti-saikosaponin a (SSa) monoclonal antibody (MAb). … The results showed that the competitive ELISA had higher sensitivity than high performance liquid chromatography (HPLC) for the detection of saikosaponin. …

Plant production science 11(2), 192-197, 2008-04-00

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LI Xiu Qin , GAO Qiu Tao , CHEN Xiao Hui , BI Kai Shun

… Saikosaponin-a and -d were converted completely into saikosaponin-b_1 and -b_2 by mild acid treatment. … Distinctive measuring of these converted diene-saponins provided a rapid and selective method for the determination of saikosaponin-a and -d in commercial samples of Radix Bupleuri. … Well resolved chromatograms of saikosaponin-b_1 and -b_2 were obtained with an isocratic elution of acetonitrile : 1% formic acid water (37.5:62.5). …

Biological & pharmaceutical bulletin 28(9), 1736-1742, 2005-09-01

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Ohno Tsutomu , Mikami Eiichi , Matsumoto Hiroshi , Oka Hisao , Nin Itsuhiro

… Saikosaponin a, c, and d could be separated from sample solutions of saiko using a 2-butanone/mcthanol/water (1:1:1) solvent system. …

Natural medicines 58(5), 218-221, 2004-10-20

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ZHU Shu-hang , SHIMOKAWA Shin-ichi , TANAKA Hiroyuki , SHOYAMA Yukihiro

… For immunization, saikosaponin a (SSa) was conjugated with bovine serum albumin (BSA). … Weak cross-reactivities occurred with saikosaponin c, b2 and d, which are stereochemical and/or functional isomers of SSa, but no crossreactivities were observed with other related steroidal glycosides. …

Biological & pharmaceutical bulletin 27(1), 66-71, 2004-01-01

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FUJIOKA Toshihiro , YOSHIDA Keisuke , FUJII Hiroko , NAGAO Tsuneatsu , OKABE Hikaru , MIHASHI Kunihide

The MeOH extract of the fruits of Bupleurum rotundifolium showed inhibitory activity against human gastric adenocarcinoma (MK-1) cell gnmth (GI_50 : 6.25 μg/ml). From this extract, 10 new ursane-type …

Chemical & pharmaceutical bulletin 51(4), 365-372, 2003-04-01

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Ishiguro Hinako , Takeuchi Miwa , Namera Akira , Watanabe Takashi , Isoda Susumu , Malla Kuber Jung , Takano Akihito , Kohda Hiroshi

… The amounts of acetylsaikosaponins, saikosaponin-a and -d in this plant were found to be comparatively higher than those in B.falcatum and B.chinensis. … However, saikosaponin-c, one of the major saponins in B.falcatum, was not detected in this plant. …

Natural medicines 55(2), 55-60, 2001-04-20

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MESELHY Meselhy R.

… A-44, two glycosidases responsible for the stepwise hydrolysis of SAPs were isolated and characterized as saikosaponin-hydrolyzing β-D-glucosidase and prosaikogenin-hydrolyzing β-D-fucosidase. … After the oral administration of saikosaponin b_1 (Sb_1) to germ-free rats, no metabolites were detected in their plasma, cecal contents and cumulative feces. …

Journal of traditional medicines 17(1), 1-11, 2000-02-25

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