Search Results:　 1-18 of 18

1

Preparation of Monoclonal Antibodies Reactive to a Hallucinogenic Drug, Psilocin

Yamaguchi Mitsune , Saito Toshiaki , Horiguchi Yoshie , Ogawa Keita , Tsuchiya Yukihiro , Hishinuma Kayoko , Chikuma Toshiyuki , Makino Yukiko , Hojo Hiroshi

… To identify these mushrooms, we attempted to prepare the monoclonal antibody (mAb) reactive to these hallucinogens. … We obtained several hybridoma cells producing mAbs reactive to psilocin, from which fourclones, BA631, CA231, KA422, and MA332 with a higher production of anti-psilocin mAb were selected by limiting dilution. …

Journal of health science 50(6), 600-604, 2004-12-01

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2

Enzyme Immunoassay for a Characteristic Protein in the Animal Crude Drug Lumbricus

BAI Gang , FUJIWARA Kunio , KAMINISHI Yoshie , LI Hao Zhi , KITAGAWA Tsunehiro

… A selected antibody enzyme immunoassay (SAEIA) was developed using the antiserum and the purified 70 kDa protein as a solid-phase antigen. …

Biological & pharmaceutical bulletin 21(3), 197-201, 1998-03-15

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3

[原著］Estimation of rabbit fibrinopeptide B using a monoclonal antibody

Kinjoh Kiyohiko , Nakamura Mariko , Nejime Tetsuya , Tokeshi Yoshihiro , Kosugi Tadayoshi , First Department of Physiology Faculty of Medicine University of the Ryukyus. Okinawa Japan

… We produced a monoclonal antibody against rabbit fibrinopeptide B (FPB) and established an immunological assay system to estimate it. … As a result, one hybridoma (IA12/4A2) was selected, which secreted an antibody against rabbit FPB. … The monoclonal antibody obtained belonged to the IgM class and its light chain consisted of a $ \kappa $-chain. …

琉球医学会誌 = Ryukyu Medical Journal 18(4), 129-133, 1998-00-00

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4

Development and Application of a Sandwich Enzyme Immunoassay for Glycyrrhizae Radix Protein (GRP) Using Monoclonal Antibodies

BAI Gang , FUJIWARA Kunio , TANIMORI Hideaki , KITAGAWA Tsunehiro

… Among these, anti-GRP mAb-1 and 2 were found to recognize different epitopes on the GRP molecule, as demonstrated by ELISA analysis, and were used for the development of a sandwich enzyme immunoassay (SEIA) for GRP in traditional Chinese medicines (TCMs). …

Biological & pharmaceutical bulletin 20(12), 1224-1228, 1997-12-15

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5

Application of a Licorice Root Specific Protein to a General Method for the Assay of Licorice Root Components in Traditional Chinese Medicines

KITAGAWA Tsunehiro , BAI Gang , FUJIWARA Kunio , AKAHORI Akira , SONOI Sinsuke , KONDO Seizo , LI Hao Zhi

… A new fuzzy immunoassay method generally applied to ten Glycyrrhizae Radix (GR) preparations of four different botanical origins was studied. … The presence of the characteristic GR protein (GPR) was shown using Western blot analyses and selected antibody enzyme immunoassay (SAEIA) methods. … A GRP was isolated from one of the GR specimens which was selected using SAEIA methods. …

Biological & pharmaceutical bulletin 20(6), 589-595, 1997-06-15

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6

A General Enzyme Immunoassay for the licorice Root Component Contained in Traditional Chinese Medicines

KITAGAWA Tsunehiro , YANG Zhi , BAI Gang , FUJIWARA Kunio , AKAHORI Akira , SONO Shinsuke , KONDO Seizo

… The development and application of a new enzyme immunoassay for general assay of the Glycyrrhizae radix (GR) component in Chinese traditional medicines is described. … The proportion of antibody binding to the solid-phase GRSK component wa detected using na enzyme-labeled second antibody. …

Biological & pharmaceutical bulletin 20(3), 211-216, 1997-03-15

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7

A New Method for the Detection and Quantitative Measurement of the Contents of Trichosanthes Root Component Composing Chinese Traditional Medicines

KITAGAWA Tsunehiro , BAI Gang , FUJIWARA Kunio , AKAHORI Akira , SONOI Shinsuke , KONDO Seizo

… Two selected antibody enzyme immunoassay (SAEIA) methods, the SAEIA A for assay of TR extract and the SAEIA B for assay of karasurin A were used as detection methods for the separation of TR antigens. … A SAEIA C method for assay of TR glycan component was developed using biotinylated second antibody and peroxidase-labeled avidin as the detection method. …

Biological & pharmaceutical bulletin 19(6), 783-790, 1996-06-15

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8

Specificities of Five Kinds of Antisera Produced against Crude Drugs, Pinellia Tuber, Hoelen, Glycyrrhizae Radix, Trichosanthes Root and Panax Ginseng

KITAGAWA Tsunehiro , BAI Gang , FUJIWARA Kunio

… Specificity of each antiserum was demonstrated by two immunological methods for analyses, the selected antibody enzyme immunoassay (SAEIA) and western blotting, and five crude drug extracts as specimens. …

Biological & pharmaceutical bulletin 19(3), 335-340, 1996-03-15

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9

A Visual Membrane Immunoassay for the Detection of Methamphetamine Using an Enzyme-Labeled Tracer Derived from Methamphetamine and Amphetamine

CHOI Myung Ja , GOROVITS Boris M. , CHOI Jeongeun , SONG EunYoung , NAM Kyung Soo , PARK Jongsei

… A visual membrane enzyme immunoassay is described for the measurement of methamphetamine in urine. … To increase assay sensitivity, tracers with chemically similar structures were cross-checked with the antibodies to determine their influence on the antibody binding. … Significant differences in antibody specificity were found between the two tracers. …

Biological & pharmaceutical bulletin 17(7), 875-880, 1994-07-15

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10

Development and Application of an Enzyme Immunoassay for Karasurin A, an Effective Protein Component of Trichosanthes kirilowii MAX. var. japonicum KITAM.

KITAGAWA Tsunehiro , YOSIDA Masayo , HU Jiango , SAKAI Atsuko , BAY Gang , FUJIWARA Kunio , OGIHARA Yukio , TAKEDA Tadahiro

… Rabbit antibody specific for karasurin A was identified in anti-karokon serum by the Western blotting method. …

Chemical & pharmaceutical bulletin 42(3), 599-603, 1994-03-15

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11

A New Enzyme Immunoassay for a Solid Chinese Crude Drug, Pinellia Tuber

TANIMORI Hideaki , AKAHORI Akira , SONOI Shinsuke , KITAGAWA Tsunehiro

… A new immunoassay for a solid Chinese crude drug was studied. …

Chemical & pharmaceutical bulletin 39(6), 1476-1479, 1991-06-25

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12

Development and Application of a New Method for Assay of the Content of Hoelen Extract in Chinese Traditional Medicines

TANIMORI Hideaki , MAEDA Takako , AKAHORI Akira , SONOI Shinsuke , KONDO Seizo , KITAGAWA Tsunehiro

… A newly selected antibody enzyme immunoassay (SAEIA) for aqueous hoelen extract was developed. …

Chemical & pharmaceutical bulletin 38(11), 3064-3068, 1990-11-25

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13

A NEW METHOD FOR THE QUANTITATIVE ANALYSIS OF THE AQUEOUS EXTRACT OF PINELLIA TUBER CONTAINED IN SEVERAL CHINESE TRADITIONAL MEDICINES

KITAGAWA Tsunehiro , TANIMORI Hideaki , KOHNO Yohko , AKAHORI Akira , SONOI Shinsuke , KONDO Seizo

… A new method for enzyme immunoassay of the tuber extract was developed with an antiserum to the tuber and a solid-phase antigen as immunological reagents, and β-D-galactosidase-labeled anti-rabbit IgG as the tracer. …

Chemical & pharmaceutical bulletin 37(4), 1134-1136, 1989-04-25

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14

Purification of Two Specific Antibodies against Drug and Carrier Protein Molecules(Biological)

TANIMORI HIDEAKI , YOSHIDA KENSEI , MOTOMURA HIDEAKI , KITADA KAZUHIRO , YAGISAWA SIROKI , KITAGAWA TSUNEHIRO

… Affinty purification procedures for antibodies specific to a drug and to a carrier protein were examined in detail with the use of three enzyme immunoassays (EIAs). … The influence of the spacer on the affinity purification of an antibody specific to BLS was quantitatively analyzed. … The antibody showed higher binding to BLS bound to the solid matrix with a carrier protein than without. …

Chemical & pharmaceutical bulletin 35(5), 2062-2070, 1987-05-25

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15

A MONOCLONAL ANTIBODY TO A TESTICULAR CHORIOCARCINOMA CELL LINE [in Japanese]

Yamazaki Haruki , Machida Toyohei

睾丸胚細胞性腫瘍における新しい免疫学的な診断・治療法を開発する目的で睾丸腫瘍細胞株に対するモノクローナル抗体を作製した.これはヒト睾丸絨毛上皮癌細胞株を免疫したBALB/cマウスの脾細胞とマウス骨髄腫SP2/O-Ag14を細胞融合したハイブリドーマより得られた.ハイブリドーマ抗体のスクリーニングおよび特異性の検討は,絨毛上皮癌細胞株をふくめた13種のヒト培養細胞株を標的とした酵素免疫測定法(EIA …

The Japanese Journal of Urology 78(5), 802-807, 1987-05-20

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16

Human Serum Thyroglobulin Determination Monoclonal Antibody One-step Assay:Minimum Interference of Autoantibody

KATO RYOJI , NOGUCHI SHIRO , NOGUCHI AKITO

… Combination of two thyroglobulin monoclonal antibodies (monoAbs) recognizing epitopes which are rarely recognized by an antibody enabled us to develop a rapid one-step enzyme immunoassay of serum Tg. … Of 87 monoclonal antibodies, 20 were selected for the purpose. …

Endocrinologia Japonica 34(2), 171-178, 1987

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17

Identification of 19-nor-deoxycorticosterone in normal rat serum by enzyme immunoassay and gas chromatography-mass spectrometry.

KOBAYASHI YOSHIHARU , OGIHARA TOSHIO , YAMAMURA YAYOI , WATANABE FUKUKO , KIGUCHI TOSHIKO , NINOMIYA ICHIYA , KUMAHARA YUICHI

… The present study was undertaken to investigate the presence of 19-nor-deoxycorticosterone in normal rat serum both by a sensitive enzyme immunoassay and by gas chromatography-mass spectrometry (GC/MS).<BR>19-Nor-deoxycorticosterone in rat serum was separated from other steroids prior to enzyme immunoassay by using high performance liquid chromatography (HPLC). …

Endocrinologia Japonica 31(1), 49-53, 1984

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18

Glycyrrhetylamino Acids : Synthesis and Application to Enzyme Immunoassay for Glycyrrhetic Acid

KANAOKA MATAO , YANO SABURO , KATO HIROMI , NAKANO NAOKO

… Glycyrrhetylamino acids (5a-c) were prepared by the condensation of glycyrrhetic acid (GA) with amino acids (glycine, γ-aminobutyric acid, and ε-aminohexanoic acid), which were selected for use as chemical bridges between the hapten and carrier protein in an enzyme immunoassay (EIA) for GA. … β-Galactosidase was used as the labeled enzyme and was conjugated with GA by the N-hydroxysuccinimide ester method. …

Chemical & pharmaceutical bulletin 29(6), 1533-1538, 1981-06-25

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