Search Results:　 1-20 of 31

Nakayama A. , Haraguchi M. , Tsujimoto H. , Fukushima M. , Higuchi I. , Kuribayashi H. , Utsumi H. , Hirato J. , Hashizume Y. , Akiyama S.

Congenital anomalies 42(3), 248-249, 2002-09-00

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OKUYAMA Kiyoshi , HAMAMOTO Tomoki , NOGUCHI Toshitada , MIDORIKAWA Yuichiro

… The pyrimidine nucleoside phosphorylase (Py-NPase) of Bacillus stearothermophilus TH 6-2 is a dimer of 46-kDa subunits and catalyzes the reversible phosphorolysis of uridine and thymidine. … The gene encoding this pyrimidine nucleoside phosphorylase (pyn gene) has been cloned and sequenced from B. …

Bioscience, biotechnology, and biochemistry 60(10), 1655-1659, 1996-10-23

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Takehara Munenori , Ling Feng , Izawa Shingo , Inoue Yoshiharu , Kimura Akira

… Both the purine nucleoside phosphorylase (PNPase) and uridine phosphorylase (UPase) of this bacterium were induced simultaneously when the bacterium was cultured in a medium containing adenosine or uridine as a sole source of carbon and nitrogen. …

Bioscience, biotechnology, and biochemistry 59(10), 1987-1990, 1995-10-23

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SHIRAE Hideyuki , YOKOZEKI Kenzo

… An orotidine-phosphorolyzing enzyme and a purine nucleoside phosphorylase (PNPase) of Erwinia carotovora AJ 2992, which is a potent producer of ribavirin (1-β-D-ribofuranosyl-1,2,4-triazole-3-carboxamide), an antiviral agent, from orotidine and 1,2,4-triazole-3-carboxamide (TCA), were purified 23-fold and 103-fold, respectively. …

Agricultural and biological chemistry 55(7), 1849-1857, 1991-07-23

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HORI Nobuaki , WATANABE Mutsumi , YAMAZAKI Yoshinari , MIKAMI Yoichi

… A thermostable pyrimidine nucleoside phosphorylase has been purified from Bacillus stearothermophilus JTS 859. … The Michaelis constants for 5-methyluridine, uridine, thymidine, and 2'-deoxyuridine were 0.32, 0.19, 0.46, and 0.58mM, respectively. …

Agricultural and biological chemistry 54(3), 763-768, 1990-03-23

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DOI Muneharu , TSUNEMI Yutaka , ASAHI Satoru , AKIYAMA Shun-ichi , NAKAO Yoshio

… This work was designed to obtain a microbial mutant suitable for the industrial production of uridine. … Among them, strain No. 508 produced 46mg/ml of uridine; … When this strain was subjected to further mutation to obtain mutants deficient in uridine phosphorylase activity, another mutant, No. 556, was obtained that produced 55 mg/ml of uridine with no appreciable amount of uracil. …

Agricultural and biological chemistry 52(6), 1479-1484, 1988-06-23

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MIWA MASANORI , COOK ALAN , ISHITSUKA HIDEO

… The antitumor agent 5'-deoxy-5-fluorouridine (5'-dFUrd) is a prodrug from which 5-fluorouracil (5-FUra) is generated mainly by uridine phosphorylase in mice and by thymidine phosphorylase in human tumors. …

Chemical & pharmaceutical bulletin 34(10), 4225-4232, 1986-10-25

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