Effect of Docosahexaenoic Acid on Lipoprotein Synthesis and Secretion by Cultured Eel Hepatocytes.

• Ndiaye Jibril

  The United Graduate School of Agricultural Science, Kagoshima University

• Hayashi Seiichi

  Laboratory of Food Chemistry, Faculty of Fisheries, Kagoshima University

書誌事項

タイトル別名

• Effect of Docosahexaenoic Acid on Lipop

抄録

We investigated effect of docosahexaenoic acid on lipoprotein synthesis and secretion by cultured eel hepatocytes. When eel hepatocytes were incubated with 1 mM docosahexaenoic acid (DHA) at 28°C for 24 h, triacylglycerol (TG), free cholesterol (FC), and cholesteryl ester (CE) syntheses from ¹⁴C-acetate increased 9.7-, 1.6-, and 8.5-fold, respectively, and the specific activity of TG was twofold that of control. These results indicate the stimulative effect of DHA on lipid synthesis. However, the radioactivity of the lipoprotein secreted by DHA-treated cells incubated with ¹⁴C-acetate and 3H-leucine reduced to 55 and 60%, respectively, compared to that by control cells, but DHA did not affect the incorporation of ³H-leucine into other secreted protein. Furthermore, the amount of the secreted lipoprotein per 24 h by DHA-treated cells also reduced to 17%. These results show that DHA inhibits the secretion of lipoprotein. Intracellular lipids such as TG, PL, and TC increased by 4.8, 1.6, and 1.8 times, respectively, in DHA-treated cells. The remarkable increase in TG in DHA-treated cells seems to be due to the stimulative effect of DHA on TG synthesis and an inhibitory effect of DHA on lipoprotein secretion, since about 70% of the secreted lipoprotein consists of TG. The chemical composition and apoprotein profile of the lipoprotein secreted by DHA-treated and control cells were almost the same. These results suggest that DHA inhibits the assembly or secretion of the lipoprotein in eel hepatocytes.

収録刊行物

• Cell Structure and Function

  Cell Structure and Function 21 (5), 307-315, 1996

  日本細胞生物学会

キーワード

• eel
• cultured hepatocytes
• docosahexaenoic acid
• lipoprotein

詳細情報

• CRID: 1390282679671628160
• NII論文ID: 130003512953
• NII論文ID: 10001914774
• NII書誌ID: AA0060007X
• DOI: 10.1247/csf.21.307
• COI: 1:CAS:528:DyaK2sXpt1elsw%3D%3D
• ISSN: 13473700
• ISSN: 03867196
• NDL書誌ID: 4131861
• PubMed: 9118236
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4131861
• Web Site: http://www.jstage.jst.go.jp/article/csf1975/21/5/21_5_307/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可