低温下での蛋白質X線結晶解析技術 [in Japanese] Methods in Cryogenic X-ray Protein Crystallography [in Japanese]
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X-ray prortein crystallography is now applied to the analysis of large protein complex system, however, there still exists a problem in its application, that is X-radiation damage of protein crystals. To reduce radiation damage, cryogenic experiment at liquid nitrogen temperature is the best way. In order to utilize the method, some experimental procedures have to be developed; addition of anti-freezing reagent, crystal mounting device, rapid cooling method. Here, the author descrives the important aspects of each procedure and some results of cryogenic experiments.
X-RAYS 37(5), 266-270, 1995-10-31
The Crystallographic Society of Japan