低温下での蛋白質Ｘ線結晶解析技術

• 中迫 雅由

  理化学研究所生物物理研究室

書誌事項

タイトル別名

• Methods in Cryogenic X-ray Protein Crystallography.
• テイオンカ デ ノ タンパクシツ Xセン ケッショウ カイセキ ギジュツ

抄録

X-ray prortein crystallography is now applied to the analysis of large protein complex system, however, there still exists a problem in its application, that is X-radiation damage of protein crystals. To reduce radiation damage, cryogenic experiment at liquid nitrogen temperature is the best way. In order to utilize the method, some experimental procedures have to be developed; addition of anti-freezing reagent, crystal mounting device, rapid cooling method. Here, the author descrives the important aspects of each procedure and some results of cryogenic experiments.

収録刊行物

• 日本結晶学会誌

  日本結晶学会誌 37 (5), 266-270, 1995

  日本結晶学会

詳細情報

• CRID: 1390282679063598080
• NII論文ID: 10002024425
• NII書誌ID: AN00188364
• DOI: 10.5940/jcrsj.37.266
• ISSN: 18845576
• ISSN: 03694585
• NDL書誌ID: 3636229
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3636229
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可