3. 新しいタンパク質立体構造 Paracoccus denitrificans 由来チトクローム酸化酵素の2.8@90分解能におけるX線結晶構造解析 [in Japanese] Structure of Cytochrome c Oxidase from Paracoccus denitrificans at 2.8@90 Resolution [in Japanese]
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The crystal structure of cytochrome <I>c</I> oxidase from <I>Paracoccus denitrificans</I> at 2.8Å resolution is described. The crystallization of this membrane protein complex is achieved by co-crystallization with antibody Fv-fragment. The enzyme contains four subunits. Subunit I contains twelve tranmembrane helices and binds heme <I>a</I> and the heme <I>a</I><SUB>3</SUB>-copper B binuclear center where molecular oxygen is reduced to water. Two proton transfer pathways, one for protons consumed in water formation and one for'proton pumping'could be identified. A possible mechanism for proton pumping is discussed.
X-RAYS 38(1), 41-47, 1996-02-28
The Crystallographic Society of Japan