3. 新しいタンパク質立体構造 マクロファージ遊走阻止因子 -セレノメチオニンを使ったMAD法による解析例- [in Japanese] Structure Analysis of Macrophage Migration Inhibitory Factor [in Japanese]
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Structure of macrophage migration inhibitory factor has been determined by the method of multiwavelength anomalous diffraction (MAD) with the use of synchrotron data from a crystal of the selenomethionyl protein. The protein contains three methionines in a single polypeptide chain of 114 amino residues. In the analysis, we prepared a series of selenomethionyl proteins by site-directed mutagenesis. The structure was solved using the crystal which contains only one methionine (therefore one Se) per single polypeptide chain.
X-RAYS 38(1), 54-59, 1996-02-28
The Crystallographic Society of Japan