Protein Crystallography and Structural Biology. Hot 3D Structures of Biological Macromolecules. Structure Analysis of Macrophage Migration Inhibitory Factor.

• SUZUKI Mamoru

  Photon Factory, National Laboratory for High Energy Physics

• SUGIMOTO Hiroshi

  Division of Biological Sciences, Graduate School of Science, Hokkaido University

• NAKAGAWA Atsushi

  Division of Biological Sciences, Graduate School of Science, Hokkaido University

• TANAKA Isao

  Division of Biological Sciences, Graduate School of Science, Hokkaido University

Bibliographic Information

Other Title

• タンパク質結晶学と構造生物学　新しいタンパク質立体構造　マクロファージ遊走阻止因子　セレノメチオニンを使ったＭＡＤ法による解析例
• マクロファージ ユウソウ ソシ インシ セレノメチオニン オ ツカッタ MAD

Abstract

Structure of macrophage migration inhibitory factor has been determined by the method of multiwavelength anomalous diffraction (MAD) with the use of synchrotron data from a crystal of the selenomethionyl protein. The protein contains three methionines in a single polypeptide chain of 114 amino residues. In the analysis, we prepared a series of selenomethionyl proteins by site-directed mutagenesis. The structure was solved using the crystal which contains only one methionine (therefore one Se) per single polypeptide chain.

Journal

• Nihon Kessho Gakkaishi

  Nihon Kessho Gakkaishi 38 (1), 54-59, 1996

  The Crystallographic Society of Japan

Details

• CRID: 1390001204087167616
• NII Article ID: 10002024740
• NII Book ID: AN00188364
• DOI: 10.5940/jcrsj.38.54
• ISSN: 18845576
• ISSN: 03694585
• NDL BIB ID: 3934771
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3934771
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed