4. 周辺科学との協調とその将来 有機化学とタンパク質結晶学 -遷移状態アナログを用いて酵素反応機構を探る- [in Japanese] Organic Chemistry and Protein Crystallography Probing Enzyme Mechanisms with Transition State Analogs [in Japanese]
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The purpose of this review is to describe what is deduced about the source of the catalytic power for enzymes, using as guides the crystal structures of the enzymes complexed with transition-state analogs. We start with an overview of transition-state theory and its application to enzyme-catalyzed reactions. We proceed to consideration of the transition-state analogs as probes for the interactions that occur at enzyme active sites. Finally, we describe the case study in an enzyme, glutathione synthetase, and consider a practical approach to enzyme mechanisms using transition-state analogs.
X-RAYS 38(1), 99-104, 1996-02-28
The Crystallographic Society of Japan