Localization of 47 kD Antigenic Polypeptides in Malaria Parasites by Confocal Laser Scanning Microscopy
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- Kano Shigeyuki
- Department of Parasitology, Gunma University School of Medicine
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- Nakamura Norio
- Department of Parasitology, Gunma University School of Medicine
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- Murakami Tohru
- Department of Anatomy, Gunma University School of Medicine
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- lshikawa Harunori
- Department of Anatomy, Gunma University School of Medicine
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- Suzuki Mamoru
- Department of Parasitology, Gunma University School of Medicine
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The 47 kD molecule of the asexual blood stage of Plasmodium falciparum (P. f) was reported to react specifically with sera from patients at the acute stage of infection (Kano et al., 1990). Confocal laser scanning microscopy was applied to the indirect fluorescent antibody test (IFAT) in order to observe the localization of the 47 kD antigenic polypeptides. Two monoclonal antibodies (mAbs) which were observed to bind specifically to the 47 kD molecule were prepared. Optical section images of the confocal micrographs of the IFAT demonstrated that these two mAbs against the same 47 kD molecule reacted with different sites within the parasites: one showed the reaction in the center of the merozoite; the other, on the surface of the merozoite. The spatial distribution of antigens were the same in geographically different Gambian and Colombian strains and Thailand-Myanmer border isolate. It was thus revealed that the 47 kD molecule consists of at least two differentiable antigenic polypeptides. Confocal laser scanning microscopy in the study of reactivities of mAbs with subcellular structures was useful for determining the localization of the antigenic molecules with the least possible modification in the parasite.
収録刊行物
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- bioimages
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bioimages 3 (1), 13-17, 1995
日本バイオイメージング学会
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詳細情報 詳細情報について
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- CRID
- 1390574732433945344
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- NII論文ID
- 10002037454
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- NII書誌ID
- AA11084187
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- ISSN
- 09192719
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可