Estimation and Analysis of Relative Amounts of Murine Attachmin Expressed in Cell Membranes of a Monocyte/Macrophage Lineage

• Tomita Mitsuko

  Department of Biochemistry, Saitama Medical School

抄録

Immunostaining and confocal laser microscopy were used to estimate relative amounts of murine attachmin in the macrophage plasma membranes at various periods of cell differentiation, i. e., M1, PU5-1.8, J774.1, TtT/M-87, peritoneal inflammatory macrophages, and resident macrophages. The results demonstrated that the various cells used in this study expressed attachmin in the cell membranes. However, the amount of attachmin was very different from one kind of cell to another. The strength of nonspecific adhesion of macrophages was almost proportional to the amount of attachmin in the cell membranes. It is suggested that the amount of attachmin in macrophages is available as a new differentiation marker for cells.

収録刊行物

• bioimages

  bioimages 3 (2), 107-112, 1995

  日本バイオイメージング学会

キーワード

• attachmin
• monocyte/macrophage lineage
• nonspecific adhesion protein
• glass-adherent protein
• quantitative analysis
• confocal laser microscopy

詳細情報

• CRID: 1390856351209374848
• NII論文ID: 10002037615
• NII書誌ID: AA11084187
• DOI: 10.11169/bioimages.3.107
• ISSN: 09192719
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可