High-Speed Confocal Fluorescence Microscopy Using a Nipkow Scanner with Microlenses for 3-D Imaging of Single Fluorescent Molecule in Real Time

• lchihara Akira

  Yokogawa Research Institute Corp.

• Tanaami Takeo

  Sensor Laboratory, Yokogawa Electric Corp.

• lsozaki Katsumi

  Sensor Laboratory, Yokogawa Electric Corp.

• Sugiyama Yumiko

  Sensor Laboratory, Yokogawa Electric Corp.

• Kosugi Yasuhito

  Sensor Laboratory, Yokogawa Electric Corp.

• Mikuriya Kenta

  Sensor Laboratory, Yokogawa Electric Corp.

• Abe Michio

  Department of Biology, Tokyo Metropolitan University

• Uemura lsao

  Department of Biology, Tokyo Metropolitan University

Abstract

Recent developments in confocal fluorescence microscopy have made 3-D observation of biological specimens possible, an epoch-making event in the long history of microscopy. However, most confocal microscopes currently used are insufficient for the observation of living cells and organs, which require real-time observations with minimal fluorescence staining. We have developed an innovative Nipkow-type scanner for a confocal microscope in which a microlens array is placed in front of a pinhole array and thus the optical efficiency of high-speed scanning is improved. Scanning speeds of 1,000 frames/sec are attained with 10—1000 times greater S/N than those of conventional confocal microscopes.

Journal

• bioimages

  bioimages 4 (2), 57-62, 1996

  Bioimaging Society

Keywords

• confocal fluorescence microscope
• Nipkow scanner
• microlens
• real-time 3-D imaging
• living cell observation

Details

• CRID: 1390852271221653504
• NII Article ID: 10002037699
• NII Book ID: AA11084187
• DOI: 10.11169/bioimages.4.57
• ISSN: 09192719
• Text Lang: en
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed