SEM Imaging of Single Heavy Meromyosin Molecules on Hydrophilic Silicon Surface
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- Furuno Taiji
- Department of Physics, School of Medicine, Keio University
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- Miyata Hidetake
- Department of Physics, Faculty of Science and Technology, Keio University
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- Yoshikawa Hiroshi
- Department of Physics, Faculty of Science and Technology, Keio University
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- Kinosita Jr. Kazuhiko
- Department of Physics, Faculty of Science and Technology, Keio University
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- lkegami Akira
- Department of Physics, School of Medicine, Keio University
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- Sasabe Hiroyuki
- The Institute of Physical and Chemical Research (RIKEN)
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- Knoll Wolfgang
- The Institute of Physical and Chemical Research (RIKEN)
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Abstract
The fine structure of skeletal muscle heavy meromyosin (HMM) with two heads connected to a thin tail was clearly imaged with a high-resolution scanning electron microscope (SEM) by negative staining on a hydrophilic silicon surface. The single molecular image of HMM was analyzed. A structural change in the HMM head on binding of adenosine triphosphate (ATP) was detected. The HMM molecules with head(s) bent at 6-8 nm from the head-tail junction directed toward the end of the tail were found frequently in the presence of ATP. The estimated value for the bending angle was about 20° on average.
Journal
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- bioimages
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bioimages 4 (2), 73-78, 1996
Bioimaging Society
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Details
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- CRID
- 1390007846293106688
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- NII Article ID
- 10002037729
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- NII Book ID
- AA11084187
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- ISSN
- 09192719
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- Text Lang
- en
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed