Chemical Bleaching Improves the Fluorescence Detection Efficiency of Fungi Possessing Dark-Brown Pigments

• Kawasaki Yukishige

  1Mitsubishi Kasei Institute of Life Sciences Institute of Space and Astronautical Sciences

• Tsuji Takashi

  1Mitsubishi Kasei Institute of Life Sciences

• Morimoto Michiko

  Faculty of Science, Toho University

• Haruyama Jun'ichi

  Institute of Space and Astronautical Sciences

• Takeshima Seiji

  Faculty of Agriculture, Tamagawa University

• Mikawa Takashi

  Mitsubishi Chemical Industry

抄録

Fungi possessing dark-brown pigments such as plant melanin are difficult to detect by fluorescence microscopic techniques. Pretreatment of the pigmented fungi with oxides or chlorides improved the detection efficiency by the fluorescence microscopic technique. Chemical bleaching was effective for spores of all the fungi studied. The fluorescence intensity of probes for cytoplasmic membranes, nucleic acids, and esterases stained to the spores increased more than ten times by this pretreatment, and became detectable not only with an electronic camera but also with the eye.

収録刊行物

• bioimages

  bioimages 4 (2), 85-88, 1996

  日本バイオイメージング学会

キーワード

• fluorescence microscopy
• microorganisms
• bleaching
• fungi
• pigments

詳細情報

• CRID: 1390289321270566144
• NII論文ID: 10002037759
• NII書誌ID: AA11084187
• DOI: 10.11169/bioimages.4.85
• ISSN: 09192719
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可