Whole-mount Imaging of Middle Stage Mouse Embryos by Stage-scanning Confocal Microscopy

• Kanou Yasuhiko

  Division of Molecular and Cellular Adaptation, Research Institute of Environmental Medicine, Nagoya University

• Kokubun Takuya

  Pharmacia Biotech K.K.

• Fujishita Mariko

  Meridian Instruments Far East K. K.

抄録

Whole-mount staining of embryos is a convenient way to obtain overall and three-dimensional information of the distribution of developmentally important molecules in embryos. The signals from structures, however, frequently overlap when observed by stereo microscopy using enzyme-linked antibody staining techniques. In an attempt to resolve the various structures within mouse embryos, we used a stage scanning confocal microscope. Conditions for stage scanning of whole-mount stained mouse embryos were established, and a rostrocaudal whole image of the sagittal plane was obtained.

収録刊行物

• bioimages

  bioimages 5 (2), 77-79, 1997

  日本バイオイメージング学会

キーワード

• stage scanning
• whole-mount immunohistochemistry
• mouse
• embryo
• NCAM

詳細情報

• CRID: 1391693801397668992
• NII論文ID: 10002038188
• NII書誌ID: AA11084187
• DOI: 10.11169/bioimages.5.77
• ISSN: 09192719
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可