Intensity Changes in ¹H-NMR Micro-images of Plant Materials Exposed to Subfreezing Temperatures

• Price William S.

  Water Research Institute

• lde Hiroyuki

  Water Research Institute

• lshikawa Masaya

  Department of Genetic Resources, National Institute of Agrobiological Resources

• Arata Yoji

  Water Research Institute

抄録

NMR micro-imaging provides a powerful tool for studying the changes in biological systems that occur upon cooling to subfreezing temperatures. Since the method is non-invasive, the changes can be followed in the one sample so that any dynamic processes that occur may be observed. In the present work the freezing behaviours (of water) in a wintering maple stem, a potato stem and pure water as a control were followed using NMR microimaging over the temperature range of 3 to −17°C. The images provide a clear indication of the highly organized and non-random nature of the freezing behaviours in wintering plant tissues (e.g., the maple stem) as well as contrasting behaviours between the images of the pure water, potato and maple stems as the temperature was decreased. The various factors that contribute to image intensity including NMR relaxation times, spectrometer performance and NMR spin populations, and how their respective contributions change with temperature are considered in detail. An in depth understanding of these processes is important for making valid interpretations and comparisons of images of plant materials taken at different temperatures (nonfreezing and subfreezing).

収録刊行物

• bioimages

  bioimages 5 (3), 91-99, 1997

  日本バイオイメージング学会

キーワード

• cold hardiness
• freezing
• NMR micro-imaging
• NMR microscopy
• MRI
• potato
• supercooling
• water
• maple

詳細情報

• CRID: 1390004951537410432
• NII論文ID: 10002038212
• NII書誌ID: AA11084187
• DOI: 10.11169/bioimages.5.91
• ISSN: 09192719
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可