AFM Images of Cationic Liposomes Complexed with Plasmid DNA

• Kawaura Chiyo

  Faculty of Pharmaceutical Sciences, Nagoya City University

• Furuno Tadahide

  Faculty of Pharmaceutical Sciences, Nagoya City University

• Nakanishi Mamoru

  Faculty of Pharmaceutical Sciences, Nagoya City University

抄録

Cationic liposomes with a cationic cholesterol derivative are known to be useful tools for gene transfection. In the present paper we used atomic force microscopy (AFM) for studying the structure of cationic liposomes complexed with plasmid DNA. Cationic liposomes were made by a mixture of cholesteryl-3β-carboxyamidoethylenedimethylamine (I) and 1,2-dioleoyl-sn-glycero-3-phosphatidyl ethanolamine (DOPE). AFM images of free plasmid DNA (pSV2CAT) clearly showed a circular structure of plasmid DNA with a length of 1.4 μm, and those of free cationic liposomes showed small unilamellar vesicles with diameters of 200 nm. Complex formation of the cationic liposomes with plasmid DNA showed drastic morphological changes of the liposomes. The cationic liposome-DNA complex formed a necklace-like structure at a charge ratio of 0.6 (cholesterol/phosphate) and the complex formed a capsulated structure with a diameter of 1—1.5 μm at a charge ratio of 1.2, which ratio was the most appropriate for gene transfection. Thus, it seemed that this kind of capsulation was important for gene transfection mediated by the cationic liposomes.

収録刊行物

• bioimages

  bioimages 5 (4), 121-125, 1997

  日本バイオイメージング学会

キーワード

• cationic liposomes
• gene transfection
• atomic force microscopy
• cationic cholesterol
• ethidium bromide

詳細情報

• CRID: 1390852282342140160
• NII論文ID: 10002038286
• NII書誌ID: AA11084187
• DOI: 10.11169/bioimages.5.121
• ISSN: 09192719
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可