Cellulase Hydrolysis of Acetylcelluloses Differing in Dissociation.

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  • Song Zheng
    Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University
  • Hidaka Akira
    Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University
  • Nomura Akihiko
    Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University
  • Hori Teruo
    Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University

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タイトル別名
  • Cellulase Hydrolysis of Acetylcellulose

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The cellulase-hydrolysis of acetylcelluloses with acetyl substitution (DS) from 0.20 to 2.20 was assessed based on glucose and reducing end group production and changes in viscosity of substrate solution following enzymatic hydrolysis. The hydrolysis of water-soluble acetylcellulose by cellulase may be explained as due to DS, while that of water-insoluble acetylcellulose, not only DS but also the dissociation of substrate molecules brought about through solvation, swelling and dissolution of the substrate.<br>Acetylcellulose with only DS=1.56 was found to undergo hydrolysis in aqueous cellulase solution subsequent to pre-swelling with 75 vol% aqueous ethanol. Acetylcellulose with higher DS (2.07) was hydrolyzed significantly in 80 vol% aqueous acetone by encapsulate cellulase. Acetylcelluloses with relatively high DS may thus be concluded to interact well with cellulase molecules and be hydrolyzed to varying degrees through the dissociation of solid substrates in appropriate organic solvents and cellulase can be used in such a way as to prevent it from coming into contact with the organic solvent.

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  • 繊維学会誌

    繊維学会誌 54 (7), 367-373, 1998

    社団法人 繊維学会

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