Lignin Peroxidase Production by Phanerochaete Chrysosporium Immobilized on Polyurethane Foam.

• Nakamura Yoshitoshi

  Department of Chemistry and Chemical Engineering, Faculty of Engineering, Kanazawa University

• Sawada Tatsuro

  Department of Chemistry and Chemical Engineering, Faculty of Engineering, Kanazawa University

• Sungusia Mutui Godliving

  Department of Chemistry and Chemical Engineering, Faculty of Engineering, Kanazawa University

• Kobayashi Fumihisa

  Department of Chemistry and Chemical Engineering, Faculty of Engineering, Kanazawa University

• Kuwahara Masaaki

  Wood Research Institute of Kyoto University

• Ito Hiromichi

  Wood Research Institute of Kyoto University

Bibliographic Information

Other Title

• Lignin Peroxidase Production by Phanero
• Lignin peroxidase production by Phanerochaete chrisosporium immobilized on polyurethane foam

Abstract

Production of lignin peroxidase by a white-rot basidiomycete, Phanerochaete chrysosporium, was investigated experimentally using polyurethane foam as a carrier of immobilized fungal mycelia. The immobilized cell culture using polyurethane foam as a carrier of mycelia yielded significantly greater lignin peroxidase activity than the conventional stationary liquid culture. The effects of operational conditions, such as the kind and number of polyurethane foam cubes, glucose concentration and temperature, on the lignin peroxidase production were examined. Addition of 0.05% Tween 80, 1 mM veratryl alcohol and 1 mM FeSO_4-·7H₂O greatly improved the production of lignin peroxidase up to 2,700 units/ml culture medium. The lignin peroxidase activity in this culture was about three times larger than that obtained from the culture cultivated in the absence of these additives. Step change incubation lowering the temperature from 37°C to 30°C over an incubation time of three days was carried out for the large scale production of lignin peroxidase, and this incubation gave the highest lignin peroxidase activity 3,800 units/ml culture medium.

Journal

• JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

  JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 30 (1), 1-6, 1997

  The Society of Chemical Engineers, Japan

Keywords

• <I>Phanerochaete chrysosporium</I>
• Lignin-Degrading Enzyme
• Lignin Peroxidase
• Immobilized Cell Culture
• Polyurethane Foam

Details

• CRID: 1390282679542900736
• NII Article ID: 130000020886
• NII Article ID: 10002061974
• NII Book ID: AA00709658
• DOI: 10.1252/jcej.30.1
• COI: 1:CAS:528:DyaK2sXhsVWmsbo%3D
• ISSN: 18811299
• ISSN: 00219592
• ISSN: http://id.crossref.org/issn/00219592
• NDL BIB ID: 4163619
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4163619
• Web Site: http://www.jstage.jst.go.jp/article/jcej/30/1/30_1_1/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed