Selective Recovery of Proteins by Control of Their Surface Properties Utilizing PEG-Bound Affinity Ligands

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Selective recovery of proteins by control of their surface properties is investigated by using a ligand modified aqueous two-phase system (ATPS) and polyethylene glycol (PEG) precipitation. The ligands used were nonionic surfactant (Triton and PEG-palmitate) and PEG-bound dye (Cibacron Blue and Procion Yellow). Both the partition coefficient in ATPS and the solubility in a PEG solution of Bovine Serum Albumin (BSA) increase significantly with the addition of these ligands, because the surface net hydrophobicity of BSA is increased by the binding of these hydrophobic ligands. On the other hand, these ligands have no effect on the partition behavior and solubility of Carbonic Anhydrase (CAB) and Ovalbumin (OvA), which have no binding sites to the ligands. Control of the surface properties of protein by these specific bindings of ligands can be utilized to improve the separation efficiency in ATPS and PEG precipitation. A separation process using ligand modified ATPS and PEG precipitation is developed.

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  • Journal of chemical engineering of Japan  

    Journal of chemical engineering of Japan 31(4), 618-625, 1998-08 

    The Society of Chemical Engineers, Japan

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各種コード

  • NII論文ID(NAID)
    10002066057
  • NII書誌ID(NCID)
    AA00709658
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00219592
  • NDL 記事登録ID
    4541607
  • NDL 雑誌分類
    ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-R395
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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