Growth of Human Keratinocytes on Hydrophilic Film Support and Application to Bioreactor Culture.

• Kino-oka Masahiro

  Department of Chemical Science Engineering, Osaka University

• Prenosil Jiri E.

  Department of Chemical Engineering, ETH-Zurich

Bibliographic Information

Other Title

• Growth of Human Keratinocytes on Hydrop

Abstract

Human keratinocytes were cultured on an inert polymeric film support in serum-free medium to produce subconfluent autologous skin grafts for healing of burn wounds and chronic leg ulcers. The growth of keratinocytes in Petriperm with such a hydrophilic film bottom as a culture support was superior to that in T-flask owing to better cell adhesion. Based on this principle, an autonomous modular bioreactor, KERATOR for large scale production of skin grafts up to 5280 cm² in size was constructed. It is computer controlled, and operations such as cell seeding and medium change are automated. The cell growth rate profiles in the bioreactor and Petriperm are similar, evincing the significance of KERATOR. The harvested skin grafts consist of the polymeric film covered by subconfluent sheets of non-differentiated keratinocyte cells. Such grafts may be applied to wounds in “upside-down” fashion.

Journal

• JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

  JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 31 (5), 856-859, 1998

  The Society of Chemical Engineers, Japan

Keywords

• Human Keratinocytes
• Skin Grafts
• Bioreactor
• Tissue Culture
• Biochemical Engineering

Details

• CRID: 1390282679543851008
• NII Article ID: 10002066604
• NII Book ID: AA00709658
• DOI: 10.1252/jcej.31.856
• COI: 1:CAS:528:DyaK1cXntFWqtL0%3D
• ISSN: 18811299
• ISSN: 00219592
• NDL BIB ID: 4588697
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4588697
• Web Site: http://www.jstage.jst.go.jp/article/jcej/31/5/31_5_856/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed