Immobilization of Biological Membranes by Sonication and Freeze-Thawing

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著者

    • Gotoh Takeshi GOTOH Takeshi
    • Department of Materials-Process Engineering & Applied Chemistry for Environments, Faculty of Engineering and Resource Science, Akita University
    • Kikuchi Ken-Ichi KIKUCHI Ken-ichi
    • Department of Materials-Process Engineering & Applied Chemistry for Environments, Faculty of Engineering and Resource Science, Akita University

抄録

A rapid and convenient means of immobilizing biological membranes is described. Sonication for formation of small vesicles, diffusion of the vesicles in support beads, and freeze-thawing is carried out in an immobilized multi-enzyme system. Large amounts of brush border membranes from bovine kidney were immobilized in Sepharose CL-6B, Sephacryl S-500, and Sephacryl S-1000 and increased in that order. For the latter two beads, the amounts were further increased with repetition of freeze-thawing up to a maximum of three times. Particle distribution analysis reveals that small vesicles are enlarged by the freeze-thawing repetition, suggesting an immobilization mode in which enlarged vesicles are physically entrapped in the beads. The bead-immobilized membrane vesicles were relatively stable, exhibiting constant enzyme activities for at least 6 h under packed column operation.

収録刊行物

  • Journal of chemical engineering of Japan  

    Journal of chemical engineering of Japan 31(5), 860-863, 1998-10 

    The Society of Chemical Engineers, Japan

参考文献:  10件

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各種コード

  • NII論文ID(NAID)
    10002066621
  • NII書誌ID(NCID)
    AA00709658
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    00219592
  • NDL 記事登録ID
    4588698
  • NDL 雑誌分類
    ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-R395
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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