海産自由生活性線虫類 Prochromadorella sp. と Spiliphera sp. (Chromadorida : Chromadoridae) の培養条件下での生活史 [in Japanese] Life Cycles of two Free-living Marine Nematodes, Prochromadorella sp. and Spiliphera sp. (Chromadorida : Chromadoridae), Cultured in the Laboratory [in Japanese]
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A method established to culture a free-living nematode, Microlaimus sp., from a tidal flat was applied to free-living nematodes from subtidal mud sediments. Two Chromadorids, Prochromadorella sp. and Spiliphera sp., successfully completed their life cycle in the laboratory at a temperature of 20°C. Eggs of Prochromadorella sp. hatched as juveniles 170-190μm long 3.5-4 days after spawning. Females 900-1000μm long started to deposit eggs 30 days after hatching and they deposited 42 eggs on average in 40 days. The generation time of the species was about 34 days. Eggs of Spiliphera sp. hatched as juveniles 350-360μm long 5-6.5 days after spawning. Females 1350-1500μm long started to deposit eggs 46 days after hatching and deposited 14 to 19 eggs in 40 days. The generation time of the species was about 51 days. Since all three nematode species that were able to complete their life cycles in the laboratory are epigrowth feeders, the present culture method may be applicable to other nematodes of similar feeding habits.
- Japanese Journal of Benthology
Japanese Journal of Benthology 53(2), 89-93, 1998-12-01
JAPANESE ASSOCIATION OF BENTHOLOGY