INJURY OF Escherichia coli IN PHYSIOLOGICAL PHOSPHATE-BUFFERED SALINE INDUCED BY FAR-INFRARED IRRADIATION

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著者

    • SAWAI Jun
    • Department of Chemical Engineering, Division of Chemical & Biological Science and Technology, Tokyo University of Agriculture and Technology
    • SAGARA Koji
    • Department of Chemical Engineering, Division of Chemical & Biological Science and Technology, Tokyo University of Agriculture and Technology
    • IGARASHI Hideo
    • Department of Chemical Engineering, Division of Chemical & Biological Science and Technology, Tokyo University of Agriculture and Technology
    • HASHIMOTO Atsushi
    • Department of Chemical Engineering, Division of Chemical & Biological Science and Technology, Tokyo University of Agriculture and Technology
    • KOKUGAN Takao
    • Department of Chemical Engineering, Division of Chemical & Biological Science and Technology, Tokyo University of Agriculture and Technology
    • SHIMIZU Masaru
    • Department of Chemical Engineering, Division of Chemical & Biological Science and Technology, Tokyo University of Agriculture and Technology

抄録

Injury in <I>Escherichia coli</I> irradiated by far-infrared radiation (FIR) was examined. We used penicillin G (PCG), chloramphenicol (CP), rifampicin (RFP) and nalidixic acid (NA) as selective reagents, and tried to evaluate the injury in bacteria irradiated by FIR on the basis of the changes in the sensitivity to selective reagents. The validity of this method was examined using UV pasteurization. <I>E. coli</I> irradiated by UV became more sensitive to CP and NA, which indicated that UV irradiation damaged nueleic acid in <I>E. coli</I>. This result agrees with those demonstrated by many researchers and suggests this method is applicable to useful evaluation of the damaged part in bacteria induced by stresses. <I>E. coli</I> irradiated by FIR became more sensitive to RFP and CP, which suggests that FIR irradiation damages RNA polymerase and ribosome in <I>E. coli</I>. Though similar changes in the sensitivity were obtained in the case of thermal conductive heating, the pasteurization effect of FIR irradiation was much greater than that of conductive heating.

収録刊行物

  • Journal of chemical engineering of Japan  

    Journal of chemical engineering of Japan 28(3), 294-299, 1995-06 

    The Society of Chemical Engineers, Japan

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各種コード

  • NII論文ID(NAID)
    10002133783
  • NII書誌ID(NCID)
    AA00709658
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00219592
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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