Kinetics and Mechanism of Acetate Kinase From Bacillus Stearothermophilus.

• Ishikawa Haruo

  Department of Chemical Engineering, University of Osaka Prefecture

• Shiroshima Masahiro

  Department of Chemical Engineering, University of Osaka Prefecture

• Widjaja Arief

  Department of Chemical Engineering, University of Osaka Prefecture

• Nakajima Hiroshi

  Biochemistry Department, Unitika Research and Development Center

• Tsurutani Ryoichi

  Biochemistry Department, Unitika Research and Development Center

抄録

The initial rates of ATP regeneration catalyzed by acetate kinase (ATP: acetate phosphotransferase; EC 2.7.2.1) from Bacillus stearothermophilus were measured under a wide range of MgADP^–, acetyl phosphate, MgATP^2– and acetate concentrations. The experimental results showed that the enzyme exhibited positive co-operativity for MgATP^2– and no co-operativity for MgADP^–, acetyl phosphate and acetate. Furthermore, the initial rates of the forward reaction, in which MgATP^2– and acetate were produced from MgADP^– and acetyl phosphate, were inhibited by MgATP^2– and acetate, and those of the reverse reaction were inhibited by MgADP^– and acetyl phosphate. The initial rate data were correlated well by using the rate equation derived based on the assumption that the reaction obeys the general reaction scheme based on the Random Bi Bi mechanism, and on the previous experimental result that the enzyme behaves like a dimeric enzyme, even though it is a tetrameric enzyme.

収録刊行物

• JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

  JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 28 (5), 517-524, 1995

  公益社団法人 化学工学会

キーワード

• Biochemical Engineering
• Allosteric Enzyme
• Random Bi Bi
• Reaction Rate
• ATP Regeneration

詳細情報

• CRID: 1390001204565948160
• NII論文ID: 10002134325
• NII書誌ID: AA00709658
• DOI: 10.1252/jcej.28.517
• COI: 1:CAS:528:DyaK2MXptVCnsLk%3D
• ISSN: 18811299
• ISSN: 00219592
• ISSN: http://id.crossref.org/issn/00219592
• Web Site: http://www.jstage.jst.go.jp/article/jcej/28/5/28_5_517/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可