PURIFICATION PROCESS FOR HEAT SHOCK PROTEINS USING AQUEOUS TWO-PHASE SYSTEM AND PEG FRACTIONAL PRECIPITATION

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A simple and effective purification process for heat shock proteins (HSPs), in which PEG fractional precipitation was combined with an aqueous two-phase system (ATPS), was successfully developed based upon the proteins' molecular surface properties. Both GroEL and GroES, typical HSPs from <I>E. coli</I>, were selectively partitioned to the PEG (top) phase of the ATPS. GroEL and GroES were selectively and stepwisely concentrated from the above PEG phase by PEG fractional precipitation. In addition, the purification of GroEL and GroES as a complex by using biospecific affinity with adenosine triphosphate (ATP) was achieved. GroEL and GroES can be purified either as individual native molecules or as a 1:1 complexed state by the control of ATP addition.

収録刊行物

  • Journal of chemical engineering of Japan  

    Journal of chemical engineering of Japan 28(6), 797-802, 1995-12-01 

    The Society of Chemical Engineers, Japan

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各種コード

  • NII論文ID(NAID)
    10002135025
  • NII書誌ID(NCID)
    AA00709658
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00219592
  • データ提供元
    CJP書誌  J-STAGE 
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