キャピラリーガスクロマトグラフィーによる精製魚油中のＩＰＡ及びＤＨＡの定量　基礎的検討

• 梶島 俊樹

  日本油脂株式会社王子工場食品研究所

• 加藤 博史

  日本油脂株式会社王子工場食品研究所

• 端本 謙一

  日本油脂株式会社王子工場食品研究所

• 和田 俊

  東京水産大学水産学部食品生産学科

書誌事項

タイトル別名

• Quantitative Determination of IPA and DHA in the Refined Fish Oil by Capillary Gas Chromatography. Fundamental Study.
• キャピラリーガス クロマトグラフィーニヨル セイセイ ギョユチュウ ノ IPA
• Fundamental Study
• 基礎的検討

抄録

Icosapentaenoic acid (IPA) and docosahexaenoic acid (DHA) in five purified fish oils were determined by gas chromatography (GC).<BR>These food components have recently drawn public attention, so the results were expressed as mg/g.<BR>A DB-WAX capillary column was used for the GC, due to its greater separation efficiency. The esterification was compared using both the boron trifluoride-methanol (BF₃-methanol) method and the potassium hydroxide-methanol (KOH-methanol) method. The IPA content determined by the KOH-methanol method was found to be approximately 15% higher than by the BF₃-methanol method. The DHA content by the KOH-methanol method was also higher by 5%. Determinations of IPA and DHA by both methods showed an intra-laboratory variation of less than 2.5%.<BR>These experimental data showed that IPA and DHA in refined fish oils can be accurately determined by capillary column GC and the esterification using KOH-methanol method.

収録刊行物

• 日本油化学会誌

  日本油化学会誌 45 (4), 351-354, 1996

  公益社団法人 日本油化学会

キーワード

• icosapentaenoic acid
• docosahexaenoic acid
• fish oil esterification method
• GC analysis

詳細情報

• CRID: 1390282679068002432
• NII論文ID: 10002268899
• NII書誌ID: AN10512582
• DOI: 10.5650/jos1996.45.351
• ISSN: 18841996
• ISSN: 13418327
• NDL書誌ID: 3954796
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3954796
• Web Site: https://www.jstage.jst.go.jp/article/jos1996/45/4/45_4_351/_pdf
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可