新規ラクチドコポリマーの合成とそのプロテアーゼによる分解性

• 白浜 博幸

  広島大学工学部第三類応用化学

• 水馬 潔志

  広島大学工学部第三類応用化学

• 安田 源

  広島大学工学部第三類応用化学

書誌事項

タイトル別名

• Synthesis of New Lactide Copolymers and Their Degradability by Proteases.
• シンキ ラクチド コポリマーノ ゴウセイ ト ソノ プロテアーゼ ニヨル ブン

抄録

For improving brittleness and biodegradability of poly (L-lactide) [P (L-LA)], new lactide copolymers [P (L-LA/ (R) -MOHEL)] composed of L-lactide (L-LA) and (R) -3-methyl-4-oxa-6-hexanolide [(R) -MOHEL ; optical active new lactone] were synthesized, and enzymatic degradation of the copolymers by proteases was examined. Lactides (L-and D, L-iso-mers) and (R) -MOHEL homopolymers [P (L-LA), P (DL-LA), and P [(R) -MOHEL]] were used as reference samples.<BR>Homo-and copolymerizations were carried out at 100°C for 48h using AlEt₃-H₂O (1 : 0.75) as the catalyst. Differential scanning calorimetry (DSC) indicated the polymers obtained [except homopolymers of DL-LA, (R) -MOHEL, and copolymers containing L-LA less than ca. 40 mol %] to be crystalline. Brittleness was improved without much decrease in film melting temperature (T_m) compared to P (L-LA), provided the copolymer contained L-LA units at more than 60 mol %.<BR>Among the enzymes used [proteases (bromelain, pronase, and proteinase K) and cholesterol esterase], proteinase K showed the highest degradability (i. e., substrate specificity) for lactide homopolymers (PLAs). The degradability of amorphous P (DL-LA) by each enzyme was greater than that of crystalline P (L-LA). Proteinase K hardly degraded P [(R) -MOHEL] at all.<BR>In the case of copolymers containing L-LA units at more than 60 mol%, the degradability by proteinase K increased with content of (R) -MOHEL, since the crystallinity (or T_m) of the copolymers decreased with increase in (R) -MOHEL. Too much (R) -MOHEL decreased degradability very much [e. g., P (L-LA/ (R) -MOHEL= 29/71)].<BR>Based on changes in molecular weight and thermal properties of (co) polymers before and after degradation by proteinase K, enzymatic degradation apparently started from polymer (film) surfaces and occurred more easily in amorphous than crystalline parts.

収録刊行物

• 日本油化学会誌

  日本油化学会誌 45 (5), 459-466, 1996

  公益社団法人 日本油化学会

キーワード

• rondom copolymerization
• lactide
• optical active new lactone
• enzymatic degradation
• protease

詳細情報

• CRID: 1390282679069014784
• NII論文ID: 10002269172
• NII論文ID: 10003288939
• NII論文ID: 10003285203
• NII書誌ID: AN10512582
• DOI: 10.5650/jos1996.45.459
• ISSN: 18841996
• ISSN: 13418327
• NDL書誌ID: 3967153
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3967153
• Web Site: https://www.jstage.jst.go.jp/article/jos1996/45/5/45_5_459/_pdf
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可