水系シリカゲルカラムを用いた高速液体クロマトグラフィーによる水素添加ホスファチジルコリンの定量

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タイトル別名
  • Determination of Hydrogenated Phosphatidylcholine by High Performance Liquid Chromatography using a Silica Gel Column in an Aqueous System.
  • ミズケイ シリカゲル カラム オ モチイタ コウソク エキタイ クロマトグラフ

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A method for the convenient quantitative determination of hydrogenated phosphatidylcholine (PC), for use as a cosmetic ingredient, was developed by using high performance liquid chromatography (HPLC), equipped with a silica gel column (Senshu Scientific, AQUASIL SS-352 N) and a UV detector (205 nm). This column possesses not only the usual properties of a silica gel column but can accommodate a mobile phase containing water. In this study, the solution used as the mobile phase was methanol/0.01 M potassium dihydrogen-phosphate, citric acid buffer (pH 3.0) (95/5, by vol.) at 1.0 mL/min. Hydrogenated PC could be detected rapidly, and there was no interfering peak. Non-hydrogenated PC was also present. Linear response was noted for hydrogenated and non-hydrogenated PC at 1004000 μg/mL and 52000 μg/mL, respectively. Minimum quantification was 50 and 1 μg/mL for these samples. Theretention time of hydrogenated PC was dependent on carbon chain length of fatty acid in PC molecules.<BR>Other phospholipids such as phosphatidylinositol (PI), phosphatidylethanolamine (PE), lysophosphatidylcholine (LPC), and sphingomyelin (SPH) could also be determined independently by this method.

収録刊行物

  • 日本油化学会誌

    日本油化学会誌 45 (8), 777-780, 1996

    公益社団法人 日本油化学会

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