Ｂａｃｉｌｌｕｓ　ｓｐ．由来のホスホリパーゼＣによる大豆リン脂質の加水分解

• 鎌田 正純

  成蹊大学工学部

• 原 節子

  成蹊大学工学部

• 戸谷 洋一郎

  成蹊大学工学部

書誌事項

タイトル別名

• Hydrolysis of Soybean Phospholipids by Phospholipase C from Bacillus sp.
• Bacillus sp.ユライ ノ ホスホリパーゼ C ニヨル ダイズ リン

抄録

The hydrolysis of soybean phospholipid comprised of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI) and phosphatidic acid (PA) by a phospholipase C from Bacillus sp. was studied under various conditions.<BR>Phospholipase C hydrolyzed preferentially PC, followed by PE>PI>PA in a biphasic reaction system of diethyl ether-phosphate buffer, while in aqueous systems, it hydrolyzed preferentially PI, followed by PC>PE> PA.<BR>PC and PE was hydrolyzed more easily in an aqueous system with phosphate buffer at pH 7.0 than with Tris-HCl buffer at pH 7.5, while the hydrolysis of PI showed a little difference between the two buffers. PA was the most difficult to be hydrolyzed in the phospholipid mixture used.<BR>The hydrolysis in the biphasic system of diethylether-phosphate buffer by phospholipase C from Bacillus sp. would appear to be a simple and rapid means for concentrating PI from a phospholipid mixture.

収録刊行物

• 日本油化学会誌

  日本油化学会誌 45 (11), 1255-1259, 1996

  公益社団法人 日本油化学会

キーワード

• phospholipase C
• soy phospholipid
• phosphatidylcholine
• phosphatidylinositol
• enzyme hydrolysis

詳細情報

• CRID: 1390001204092541696
• NII論文ID: 10002271364
• NII書誌ID: AN10512582
• DOI: 10.5650/jos1996.45.1255
• ISSN: 18841996
• ISSN: 13418327
• NDL書誌ID: 4087601
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4087601
• Web Site: https://www.jstage.jst.go.jp/article/jos1996/45/11/45_11_1255/_pdf
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可