テングニシのスフィンゴ脂質　内臓部のセラミドモノヘキソシドとセラミドアミノエチルホスホネート

• 三島 芳博

  近畿大学九州工学部工業化学科

• 岸根 秀樹

  近畿大学理工学部化学教室

• 林 陽

  近畿大学理工学部化学教室

書誌事項

タイトル別名

• Sphingolipids of Hemifusus ternatanus. Ceramide Monohexosides and Ceramide Aminoethylphosphonate of the Viscera.
• テングニシ ノ スフィンゴ シシツ ナイゾウブ ノ セラミドモノヘキソシド ト
• Ceramide Monohexosides and Ceramide Aminoethylphosphonate of the Viscera
• 内臓部のセラミドモノヘキソシドとセラミドアミノエチルホスホネート

抄録

Examination was made of ceramide monohexosides (CMH) and sphingophosphonolipids (SPnL) from the viscera of Hemifusus ternatanus.<BR>H. ternatanus belongs to Prosobranchia in animal classification, and is a popular sea snail in evolutional and biological respects.<BR>Conjugated lipids were extracted with chloroform-methanol (2 : 1, by vol) following the method for simple lipids with acetone. CMH and SPnL as main sphingolipids were separated and purified using a silicic acid column. The purified sphingolipids were identified by TLC, fast atom bombardment mass spectrometry (FAB/MS), analysis of the degradation products and quantitative analysis of sugar, long chain base, phosphorus and fatty acid ester.<BR>CMH consisted primarily of galactosylceramides (Gal-Cer), according to GC analysis of sugar in the water-soluble components. SPnL consisted of ceramide aminoethylphosphonate (CAEP) and ceramide N-methylaminoethylphosphonate (CMAEP) at 73 : 27 in positive ion mode FAB/MS and 93 : 7 in negative ion mode FAB/MS.. From GC analysis, the water-soluble component was mostly 2-amino-ethylphosphonic acid (AEP).<BR>The major fatty acids of Gal-Cer were 2-hydroxyhexadecanoic (43.7%) and 2-hydroxyheptadecanoic acids (11.4%), and that of CAEP, hexadecanoic acid (64.1%). The ratio of non-hydroxy fatty acid to 2-hydroxy fatty acid was 1 : 3 in Gal-Cer and 8 : 1 in CAEP.<BR>The major long-chain bases of Gal-Cer were C₁₈-sphingatrienine (17.9%) and C₁₈-sphinga-dienine (14.4%), and of CAEP, C₁₈-sphingatrienine (17.6%) and C₁₆-sphingosine (17.2%). The long chain bases of these sphingolipids in most cases were unsaturated long chain bases.<BR>The most remarkable analytical result for the sphingolipids obtained from viscera of H. ternatanus was the finding of the large number of even-numbered unsaturated long chain bases and trienic dihydroxy bases as d18 : 3, d19 : 3 and d21 : 3.

収録刊行物

• 日本油化学会誌

  日本油化学会誌 46 (1), 39-49, 1997

  公益社団法人 日本油化学会

キーワード

• ceramide monohexoside
• ceramide aminoethylphosphonate
• unsaturated long chain bases
• sphingatrienine
• gastropod

詳細情報

• CRID: 1390282679068620800
• NII論文ID: 10002271692
• NII書誌ID: AN10512582
• DOI: 10.5650/jos1996.46.39
• ISSN: 18841996
• ISSN: 13418327
• NDL書誌ID: 4129264
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4129264
• Web Site: https://www.jstage.jst.go.jp/article/jos1996/46/1/46_1_39/_pdf
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可