高速液体クロマトグラフィーを用いたリパーゼ活性の測定

• 深津 誠

  日本大学理工学部工業化学科

• THORSTEINSON Chales T.

  Canadian Grain Commission/Grain Research Laboratory

• DAUN James K.

  Canadian Grain Commission/Grain Research Laboratory

• 田村 利武

  日本大学理工学部工業化学科

書誌事項

タイトル別名

• Determination of Lipase Activity by High Performance Liquid Chromatography
• コウソク エキタイ クロマトグラフィー オ モチイタ リパーゼ カッセイ ノ

抄録

Lipase unit is defined as that amount of enzyme needed to produce 1 μmol non-esterified fatty acid in one minute under specified conditions. Non-esterified fatty acid is traditionally measured by acid-base titration. This was done in the present study in place of High-performance liquid chromatography (HPLC) method. HPLC with a light scattering detector was conducted to measure non-esterified fatty acids to determine the activity of lipase from Candida cylindracea. HPLC result was essentially the same as that obtained by titration, the former method being more sensitive.

収録刊行物

• 日本油化学会誌

  日本油化学会誌 46 (6), 697-700,715, 1997

  公益社団法人 日本油化学会

キーワード

• lipase activity
• non-esterified fatty acid
• HPLC
• evaporative light scattering detector

詳細情報

• CRID: 1390282679070148352
• NII論文ID: 10002272815
• NII書誌ID: AN10512582
• DOI: 10.5650/jos1996.46.697
• ISSN: 18841996
• ISSN: 13418327
• NDL書誌ID: 4237607
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4237607
• Web Site: https://www.jstage.jst.go.jp/article/jos1996/46/6/46_6_697/_pdf
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可