Peroxidase Catalyzed Decoloration of Orange II Coupled with Glucose-Glucose Oxidase System

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  • グルコース-グルコースオキシダーゼ系とのカップリングによるペルオキシダーゼを触媒とするオレンジIIの退色
  • グルコース グルコースオキシダーゼケイ ト ノ カップリング ニヨル ペルオキ

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Abstract

The decoloration of Orange II catalyzed by horseradish peroxidase (HRP) was examined in the presence of glucose and glucose oxidase (GOD). Hydrogen peroxide (H2O2) formed during enzymatic reaction of glucose with GOD was continuously consumed as a substrate in the enzymatic cycle of HRP. In the HRP-catalyzed decoloration of Orange II coupled with the glucose-GOD system, the rate of decoloration Orange II was less than that in the HRP-catalyzed decoloration of Orange II with H2O2. The different initial concentrations of H2O2 may have been the reason for this, in that the H2O2 increased with reaction time in the enzymatic reaction of glucose with GOD. In coupling enzymatic reactions, the rate of decoloration Orange II was dependent on pH and concentrations of glucose and GOD. This rate showed a broad maximum at pH 8.5-9.0. And increased with glucose and GOD concentrations. The decololation rate of Orange II under optimum conditions was sufficient to inhibit the transfer of Orange II.

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