Preparation of Phosphatidylserine from Soybean Phospholipid by Cabbage Phospholipase D
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- KAMATA Masazumi
- Faculty of Engineering, Seikei University
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- HARA Setsuko
- Faculty of Engineering, Seikei University
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- TOTANI Yoichiro
- Faculty of Engineering, Seikei University
Bibliographic Information
- Other Title
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- キャベツ起源ホスホリパーゼDによる大豆リン脂質からホスファチジルセリンの調製
- キャベツ キゲン ホスホリパーゼ D ニヨル ダイズ リン シシツ カラ ホス
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Abstract
We have tried to prepare phosphatidylserine (PS) using phosphatidylcholine (PC) concentrated soybean phospholipid (PC-90) which was composed of 90% PC and 10% phosphatidylethanolamine (PE), and serine by the transphosphatidylation with phospholipase D from fresh cabbage leaves. The optimum conditions for PS preparation were determined as follow : PC-90, 0.13mmol; diethylether, 5mL; acetate buffer (pH 6.5) which contained 1M CaCl2, 3mL; phospholipase D from fresh cabbage leaves, 0.5IU. The conversion ratio from PC-90 to PS reached to 37% for 2h under the optimum condition PC, a main component of the substrate, decreased remarkably by the transphosphatidylation or the hydrolysis, but a small amount of PE was converted into PS.<BR>Transphosphatidylation activity of the enzyme was much enhanced by pre-incubation with acetate buffer at pH 5.6 for 1h. The conversion ratio from PC-90 to PS with the pre-incubated enzyme reached to 52% after 30min under the optimum condition.
Journal
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- Journal of Japan Oil Chemists' Society
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Journal of Japan Oil Chemists' Society 47 (1), 51-56,86, 1998
Japan Oil Chemists' Society
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Details 詳細情報について
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- CRID
- 1390282679068204672
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- NII Article ID
- 10002274992
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- NII Book ID
- AN10512582
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- ISSN
- 18841996
- 13418327
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- NDL BIB ID
- 4380647
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed