HPLCによるトリアシルグリセロールの立体特異分析

• 安藤 靖浩

  北海道大学水産学部海洋生物資源化学科

書誌事項

タイトル別名

• Stereospecific Analysis of Triacyl-<I>sn</I>-glycerols by HPLC
• HPLC ニ ヨル トリアシルグリセロール ノ リッタイ トクイ ブンセキ

抄録

High-performance liquid chromatography (HPLC) for stereospecific analysis of triacyl-sn-glycerols (TG) does not have the drawbacks attending enzymatic methods and involves the separation of isomeric and enantiomeric partial acylglycerols, i.e., monoacylglycerols (MG) and diacylglycerols (DG), farmed from TG. In this method, the Grignard hydrolysis of TG to partial acylglycerols and their isolation are carried out followed by the preparation of enantiomeric or diastereomeric urethane derivatives, their resolution by chiral- or achiral-phase HPLC and fatty acid analysis as methyl esters. The accuracy of this method depends on acyl group migration during analysis, differential loss in conversion, resolution of enantiomers and calculation for acyl compositions in sn-1, sn-2, and sn-3 positions. Soybean, pine seed, yew seed, and saucy oil TG have been analyzed.

収録刊行物

• 日本油化学会誌

  日本油化学会誌 47 (10), 953-962,1143, 1998

  公益社団法人 日本油化学会

キーワード

• triacyl-sn-glycerols
• stereospecific analysis
• HPLC

詳細情報

• CRID: 1390282679068150784
• NII論文ID: 10002276743
• NII書誌ID: AN10512582
• DOI: 10.5650/jos1996.47.953
• ISSN: 18841996
• ISSN: 13418327
• ISSN: http://id.crossref.org/issn/13418327
• NDL書誌ID: 4585428
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4585428
• Web Site: https://www.jstage.jst.go.jp/article/jos1996/47/10/47_10_953/_pdf
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