キラルHPLCによるキラルグリセロ脂質の分析

• 板橋 豊

  北海道大学水産学部海洋生物資源化学科

書誌事項

タイトル別名

• Analysis of Chiral Glycerolipids by Chiral Phase HPLC
• キラル HPLC ニ ヨル キラル グリセロ シシツ ノ ブンセキ

抄録

Chiral phase HPLC is a simple, sensitive and accurate method for determining the stereoconfigurations of chiral lipid components. Enantiomers and diastereomers of glycerolipids having one or two hydroxy groups in their molecules, such as diacylglycerols (DG), alkylglycerols and phosphatidylglycerols, have been successfully separated after conversion to the 3, 5-dinitrophenylurethane derivatives by HPLC on chiral stationary phases possessing a naphthyl group. Although the chromatographic resolution of enantiomeric triacylglycerols (TG) has not been carried out to date, it is possible to determine the DG moieties of TG by examining the enantiomeric sn-1, 2- and sn-2, 3-DG generated from TG by partial Grignard degradation. Chiral phase HPLC in conjunction with polar capillary GC, reversed-phase HPLC or mass spectrometry accurately indicates enantiomer compositions of natural DG. This method has been used to determine the positional distribution of aryl groups in TG molecules, positional placement of alkyl groups in glycerol ether molecules and stereospecificity of lipid related enzymes such as lipases.

収録刊行物

• 日本油化学会誌

  日本油化学会誌 47 (10), 971-981,1144, 1998

  公益社団法人 日本油化学会

キーワード

• chiral phase HPLC
• glycerolipids
• enantiomer
• diastereomer
• 3

詳細情報

• CRID: 1390001204091445376
• NII論文ID: 10002276812
• NII論文ID: 10008264896
• NII書誌ID: AN10512582
• DOI: 10.5650/jos1996.47.971
• ISSN: 18841996
• ISSN: 13418327
• ISSN: http://id.crossref.org/issn/13418327
• NDL書誌ID: 4585430
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4585430
• Web Site: https://www.jstage.jst.go.jp/article/jos1996/47/10/47_10_971/_pdf
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
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