Flow Injection Analysis of Lipid Hydroperoxides

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  • AKASAKA Kazuaki
    Department of Applied Life Science, Graduate School of Agricultural Science, Tohoku University
  • OHRUI Hiroshi
    Department of Applied Life Science, Graduate School of Agricultural Science, Tohoku University

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  • 過酸化脂質のFIAによる分析
  • カサンカ シシツ ノ FIA ニ ヨル ブンセキ

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Abstract

Flow injection methods for lipid hydroperoxides were reviewed. The sample lorded on to the system is mixed with a reagent on line followed by a reaction and detection in series. These methods make it possible to determine lipid hydroperoxides easily and rapidly. It is required that the detection methods of FIA have rapid response, high specificity, high sensitivity and free from the effect of coexistent substances. Fluorometric detection with diphenyl-1-pyrenylphosphine was one of the most suitable methods for this purpose, the FIA using this reagent made it possible to analyze lipid hydroperoxides at 10-13 mole level within 2min intervals and with almost no effects by coexistent antioxidation agents. This method was successfully applied for edible oils and some foods. Other detection methods such as a colorimetry of I3-and a chemiluminescence method also described.

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