High-Performance Liquid Chromatographic Determination of Chlorophyll Pigments in Vegetable Oils and Fats : Collaborative Study

• ENDO Yasushi

  Faculty of Agriculture, Tohoku University

• KOUZUI Hiroyuki

  Research Laboratory, The Nisshin Oil Mills, Ltd.

• SHIBUYA Naoki

  Tokyo Head Office, Japan Food Research Laboratories

• SUZUKI Kiyoji

  Yokohama Factory, Ajinomoto Co., Inc.

• TAKAHASHI Shigeyuki

  Analytical Technical Laboratory, Nippon Yuryo Kentei Kyokai

• TSUCHIDA Shinichi

  Shizuoka Office, Honen Corporation

• NAKAMURA Kazuhiro

  Research & Development Center, Showa Sangyo Co., Ltd.

Bibliographic Information

Other Title

• 植物油脂中のクロロフィル類の高速液体クロマトグラフィーによる定量法の確立と合同実験結果
• ショクブツ ユシ チュウ ノ クロロフィルルイ ノ コウソク エキタイ クロマ

Abstract

The quantification of chlorophyll (Chl) pigments such as pheophytins a and b (Pheo-a and -b), and pyropheophytins a and b (Pyro-a and -b) present in edible vegetable oils was conducted by high performance-liquid chromatography (HPLC) provided with a fluorescent detecter. A reversed phase ODS column in combination with a mixture of acetone, methanol and water (60 : 36 : 4, vol) as the mobile phase was found most effective for separating Chl pigments by HPLC. The pigments were detected at an excitation wavelength of 420nm and emission wavelength of 660nm. Subsequent to the neutralized rapeseed (canola) and soybean oils, Pheo-a and -b and Pyro-a and -b could be determined by this method at the mg/kg level. The present HPLC is thus shown quite effective for the quantification of Chl pigments in edible oils.

Journal

• Journal of Japan Oil Chemists' Society

  Journal of Japan Oil Chemists' Society 47 (11), 1225-1232,1279, 1998

  Japan Oil Chemists' Society

Keywords

• chlorophyll
• pheophytin
• pyropheophytin
• HPLC
• vegetable oil

Details

• CRID: 1390001204091460224
• NII Article ID: 10002277773
• NII Book ID: AN10512582
• DOI: 10.5650/jos1996.47.1225
• ISSN: 18841996
• ISSN: 13418327
• NDL BIB ID: 4604482
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4604482
• Web Site: https://www.jstage.jst.go.jp/article/jos1996/47/11/47_11_1225/_pdf
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed