リポソーム二分子膜の透過性に及ぼすステロイドとビタミンD<SUB>3</SUB>の影響

• 阿部 正彦

  東京理科大学理工学部 東京理科大学界面科研

• 橋崎 要

  東京理科大学理工学部

• 横内 祐美子

  東京理科大学理工学部

• 高尾 芳子

  東京理科大学理工学部

• MANOSROI Jiradei

  Faculty of Pharmacy, Chiang Mai University

• MANOSROI Aranya

  Faculty of Pharmacy, Chiang Mai University

• 酒井 秀樹

  東京理科大学理工学部 東京理科大学界面科研

• 横山 祥子

  共立薬科大学

• 山内 仁史

  東京理科大学理工学部

書誌事項

タイトル別名

• Effects of Steroids and Vitamin D<SUB>3</SUB> on the Permeability of Liposomal Bilayer Membranes
• Effects of Steroids and Vitamin D3 on the Permeability of Liposomal Bilayer Membranes

抄録

Molecular interactions between L-α-dipalmitoylphosphatidylcholine (DPPC) and steroids differing in the number of double bonds in a monolayer and bilayer were examined by measurement of surface pressure, permeability and microviscosity. The steroids used were 7-dehydrocholesterol (7-DHC), cholesterol (Chol) and β-cholestanol (β-Chol) along with Vitamin D₃ (VD₃) having an opened steroid B-ring. The limiting molecular area for the steroids in the DPPC-steroid mixed monolayer increased with the number of double bonds in the steroid ring in the order β-Chol<Chol<7-DHC. Liposomes were formed with steroids at steroid mole fractions less than 0.5 and VD₃ less than 0.3. The permeability of the liposomes containing steroids decreased with the number of double bonds in the steroid ring and steroid limiting molecular area. That of liposomes containing VD₃ with limiting molecular area exceeding that of steroids was higher than for liposomes containing steroids. The effects of steroids or VD₃ on the permeability of Liposomal bilayer membranes cannot be adequately explained based on the number of double bonds or limiting molecular area, although Demel et al. have shown a correlation between the effects of sterols on the permeability of liposomes and molecular area of sterols in monolayers. The permeability of liposomal bilayer membranes containing steroids or VD₃ was found in this study straightly decrease with microviscosity near the bilayer center. The permeability of liposomal bilayer membranes may thus be considered to related to microviscosity near the bilayer center, which can be evaluated based on fluorescence polarization determined with the fluorescent probe, 12AS.

収録刊行物

• 日本油化学会誌

  日本油化学会誌 48 (3), 215-220,259, 1999

  公益社団法人 日本油化学会

キーワード

• Liposomal bilayer membrane
• steroid
• vitamin D<SUB>3</SUB>
• permeability of membrane
• microviscosity

詳細情報

• CRID: 1390001204091926272
• NII論文ID: 10002278576
• NII論文ID: 130001015051
• NII書誌ID: AN10512582
• DOI: 10.5650/jos1996.48.215
• ISSN: 18841996
• ISSN: 13418327
• NDL書誌ID: 4685025
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4685025
• Web Site: https://www.jstage.jst.go.jp/article/jos1996/48/3/48_3_215/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1999157917
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可