リパーゼ加水分解および高速液体クロマトグラフィーによる食用油の脂肪酸, 特に不飽和脂肪酸組成の簡易分析法

• 市川 和昭

  名古屋文理短期大学

• 近藤 美恵

  名古屋文理短期大学

• 小山 吉人

  名古屋文理短期大学

書誌事項

タイトル別名

• Simple Determination of Fatty Acid Compositions of Edible Oils by Lipase Hydrolysis and High-Performance Liquid Chromatography
• リパーゼ カスイ ブンカイ オヨビ コウソク エキタイ クロマトグラフィー ニ ヨル ショクヨウアブラ ノ シボウサン トクニ フホウワ シボウサン ソセイ ノ カンイ ブンセキホウ

抄録

High-performance liquid chromatography (HPLC) using a 205 nm UV detector was evaluated for usefulness in determining fatty acid compositions, especially unsaturated fatty acid compositions, of edible oils such as perilla, soybean and safflower oils, which mainly consist of triglycerides that contain mono and polyunsaturated fatty acids. Fatty acids were obtained by lipase-hydrolysis followed by petroleum ether extraction. Sample fatty acids were analyzed by HPLC without conversion to the derivatives. Butyric acid served as the internal standard for determination of fatty acids. The results showed close agreement with those of GC. The present method is applicable to the simple determination of fatty acid compositions, especially unsaturated fatty acid compositions, of edible oils without degradation due to heating and/or the oxidative deterioration of unsaturated fatty acids.

収録刊行物

• 日本油化学会誌

  日本油化学会誌 48 (4), 331-337,364, 1999

  公益社団法人 日本油化学会

キーワード

• fatty acid composition
• edible oils
• HPLC analysis
• lipase hydrolysis
• Perilla oil

詳細情報

• CRID: 1390001204090604032
• NII論文ID: 10002278819
• NII書誌ID: AN10512582
• DOI: 10.5650/jos1996.48.331
• ISSN: 18841996
• ISSN: 13418327
• NDL書誌ID: 4698370
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4698370
• Web Site: https://www.jstage.jst.go.jp/article/jos1996/48/4/48_4_331/_pdf
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