Capillary Gas Chromatographic Determination of C27-Bile Acids in Biological Samples and Its Application to Urine of a Patient with Zellweger Syndrome

• KUROSAWA Takao

  Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• SATO Masahiro

  Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• KIKUCHI Fumihiko

  Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• TAZAWA Yusaku

  Department of Pediatrics, Akita University School of Medicine

• TOHMA Masahiko

  Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido

書誌事項

タイトル別名

• Capillary Gas Chromatographic Determination of C27-Bile Acids in Biological Samples and Its Application to the Urine of a Patient with Zellweger Syndrome.
• Capillary Gas Chromatographic Determina

抄録

Gas chromatographic determination of C₂₇-bile acids was investigated for the study of bile acid biosynthesis and related congenital disorders. The intermediate C₂₇-bile acids in the β-oxidation step and related compounds were conveniently synthesized from the corresponding C₂₄-bile acids as standard samples. The separation of methyl ester-trimethylsilyl ether (Me-TMS) and methyl ester-dimethylethylsilyl ether (Me-DMES) derivatives of the C₂₇-bile acids was carried out by capillary gas chromatography. Then methylene units of the above derivatives were measured. The Me-DMES derivatives were more efficient for the separation of C₂₇-bile acids than the Me-TMS derivatives. The calibration curves for the Me-DMES derivatives of C₂₇-bile acids showed good linearities over the range of 5-200ng; the detection limits were ca. 0.5ng (S/N=5). The relative recoveries from biological samples were 93-105% for all C₂₇-bile acids using 24- nor-chenodeoxycholic acid as an internal standard. An application to the diagnosis of Zellweger syndrome is also discussed.

収録刊行物

• Analytical Sciences

  Analytical Sciences 12 (6), 839-846, 1996

  社団法人 日本分析化学会

キーワード

• C₂₇-Bile acid
• gas chromatography
• methylene unit
• bile acid biosynthesis
• Zellweger syndrome

詳細情報

• CRID: 1390001204255703808
• NII論文ID: 130003528105
• NII論文ID: 10002409831
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.12.839
• COI: 1:CAS:528:DyaK28XnsFCht7k%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 4099076
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4099076
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.12.839.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.12.839/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci1985/12/6/12_6_839/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1997105549
• 本文言語コード: en
• データソース種別:
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