Capillary Gas Chromatographic Determination of C27-Bile Acids in Biological Samples and Its Application to Urine of a Patient with Zellweger Syndrome
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- KUROSAWA Takao
- Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
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- SATO Masahiro
- Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
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- KIKUCHI Fumihiko
- Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
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- TAZAWA Yusaku
- Department of Pediatrics, Akita University School of Medicine
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- TOHMA Masahiko
- Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
書誌事項
- タイトル別名
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- Capillary Gas Chromatographic Determination of C27-Bile Acids in Biological Samples and Its Application to the Urine of a Patient with Zellweger Syndrome.
- Capillary Gas Chromatographic Determina
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Gas chromatographic determination of C27-bile acids was investigated for the study of bile acid biosynthesis and related congenital disorders. The intermediate C27-bile acids in the β-oxidation step and related compounds were conveniently synthesized from the corresponding C24-bile acids as standard samples. The separation of methyl ester-trimethylsilyl ether (Me-TMS) and methyl ester-dimethylethylsilyl ether (Me-DMES) derivatives of the C27-bile acids was carried out by capillary gas chromatography. Then methylene units of the above derivatives were measured. The Me-DMES derivatives were more efficient for the separation of C27-bile acids than the Me-TMS derivatives. The calibration curves for the Me-DMES derivatives of C27-bile acids showed good linearities over the range of 5-200ng; the detection limits were ca. 0.5ng (S/N=5). The relative recoveries from biological samples were 93-105% for all C27-bile acids using 24- nor-chenodeoxycholic acid as an internal standard. An application to the diagnosis of Zellweger syndrome is also discussed.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 12 (6), 839-846, 1996
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204255703808
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- NII論文ID
- 130003528105
- 10002409831
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DyaK28XnsFCht7k%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 4099076
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- Web Site
- https://ndlsearch.ndl.go.jp/books/R000000004-I4099076
- https://link.springer.com/content/pdf/10.2116/analsci.12.839.pdf
- https://link.springer.com/article/10.2116/analsci.12.839/fulltext.html
- http://www.jstage.jst.go.jp/article/analsci1985/12/6/12_6_839/_pdf
- https://search.jamas.or.jp/link/ui/1997105549
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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